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Literature summary extracted from

  • Grinna, L.S.; Robbins, P.W.
    Glycoprotein biosynthesis. Rat liver microsomal glucosidases which process oligosaccharides (1979), J. Biol. Chem., 254, 8814-8818.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.106 Triton X-100 activation, required in order to measure glucosidase activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 D-glucose 5 mM: 25% inhibition Rattus norvegicus
3.2.1.106 D-mannose 5 mM: 25% inhibition Rattus norvegicus
3.2.1.106 additional information no inhibition by EDTA Rattus norvegicus
3.2.1.106 p-nitrophenyl-alpha-D-glucoside 5 mM Rattus norvegicus
3.2.1.106 p-nitrophenyl-beta-D-glucoside 5 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum rough and smooth endoplasmic reticulum Rattus norvegicus 5783
-
3.2.1.106 membrane integral membrane protein Rattus norvegicus 16020
-
3.2.1.106 membrane microsomal membranes Rattus norvegicus 16020
-
3.2.1.106 microsome localized in rough and smooth microsomes, located on the cisternal surface of microsomal vesicles Rattus norvegicus
-
-
3.2.1.106 additional information subcellular distribution and orientation Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.106 additional information no requirement for divalent cations Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Rattus norvegicus catalyzes initial step in post-glycosylation processing of cellular glycoprotein ?
-
 ?
3.2.1.106 additional information Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.106 Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose glucosidase I is a specific alpha-(1,2)-glucosidase Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.106 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O
-
 Rattus norvegicus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O catalyzes initial step in post-glycosylation processing of cellular glycoprotein Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2-lipid + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2-lipid
-
 ?
3.2.1.106 Glc3Man9GlcNAc2-peptide + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Rattus norvegicus D-glucose + Glc2Man9GlcNAc2-peptide
-
 ?
3.2.1.106 additional information catalyzes initial step in post-glycosylation processing of cellular glycoprotein Rattus norvegicus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.106 37
-
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 6.5 7.5 broad pH optimum between Rattus norvegicus