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Literature summary extracted from

  • Lazaro, J.J.; Chueca, A.; Lopez Gorge, J.; Mayor, F.
    Properties of spinach chloroplast fructose-1,6-diphosphatase (1975), Phytochemistry, 14, 2579-2583.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 EDTA stimulates Spinacia oleracea
3.1.3.11 EDTA enhances activity slightly with an optimum at 0.4-0.8 mM Spinacia oleracea

General Stability

EC Number General Stability Organism
3.1.3.11 more stable in concentrated solutions than in dilute solutions Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 Cys above 10 mM Spinacia oleracea
3.1.3.11 fructose 1,6-diphosphate above 4 mM Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 additional information
-
 additional information
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.11 chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Co2+ slight activation Spinacia oleracea
3.1.3.11 Mg2+ required Spinacia oleracea
3.1.3.11 Mg2+ optimal concentration: 10 mM Spinacia oleracea
3.1.3.11 Mn2+ slight activation Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 leaf
-
 Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.11 4°C, is the optimal temperature for enzyme storage, freeze inactivation at -18°C Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Spinacia oleracea D-fructose 6-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.11 60
-
 increasing activity up to Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.7
-
-
 Spinacia oleracea