Literature summary extracted from

Nimmo, H.G.; Tipton, K.F.
The purification of fructose 1,6-diphosphatase from ox liver and its activation by ethylenediaminetetra-acetate (1975), Biochem. J., 145, 323-334.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.3.11	8-hydroxyquinoline	activates	Bos taurus
3.1.3.11	citrate	activates	Bos taurus
3.1.3.11	EDTA	activation	Bos taurus
3.1.3.11	EDTA	required at pH 7.2 but not at pH 9.5	Bos taurus
3.1.3.11	EGTA	activates	Bos taurus
3.1.3.11	His	activation	Bos taurus
3.1.3.11	imidazole	activates	Bos taurus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.3.11	Zn2+	-	Bos taurus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.3.11	35500	-	4 * 35500, SDS-PAGE	Bos taurus
3.1.3.11	143000	-	gel filtration	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.11	Bos taurus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.3.11	-	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.3.11	liver	-	Bos taurus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.3.11	22.5	-	-	Bos taurus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.11	D-fructose 1,6-diphosphate + H2O	-	Bos taurus	D-fructose 6-phosphate + phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.3.11	tetramer	4 * 35500, SDS-PAGE	Bos taurus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.3.11	7.2	-	-	Bos taurus

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.1.3.11	6.3	10	pH 6.3: about 30% of maximal activity, pH 10.0: about 45% of maximal activity	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)