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Literature summary extracted from

  • Amachi, T.; Bowien, B.
    Characterization of two fructose bisphosphatase isoenzymes from the hydrogen bacterium Nocardia opaca 1b (1979), J. Gen. Microbiol., 113, 347-356.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 EDTA stimulates Rhodococcus opacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP 0.1 mM, 9% inhibition of isoenzyme A, complete inhibition of isoenzyme B Rhodococcus opacus
3.1.3.11 ATP
-
 Rhodococcus opacus
3.1.3.11 Mn2+ inhibition above 0.5 mM Rhodococcus opacus
3.1.3.11 NAD+ no inhibition of isoenzyme A, 67% inhibition of isoenzyme B Rhodococcus opacus
3.1.3.11 NADH 20% inhibition of isoenzyme A, 83% inhibition of isoenzyme B Rhodococcus opacus
3.1.3.11 Ribulose 1,5-diphosphate 1 mM, 48% inhibition of isoenzyme A, 3% inhibition of isoenzyme B Rhodococcus opacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.015
-
 sedoheptulose 1,7-diphosphate isoenzyme A and B Rhodococcus opacus
3.1.3.11 0.017
-
 fructose 1,6-diphosphate isoenzyme A and B Rhodococcus opacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required Rhodococcus opacus
3.1.3.11 Mn2+ can partially replace Mg2+ in activation Rhodococcus opacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 140000
-
 isoenzyme A, gel filtration Rhodococcus opacus
3.1.3.11 190000
-
 isoenzyme B, gel filtration Rhodococcus opacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O Rhodococcus opacus isoenzyme A is present when the organism is grown under autotrophic conditions but is not synthesized during heterotrophic growth. Isoenzyme B is detected in both autothrophically and heterotrophically grown organisms ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Rhodococcus opacus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 partial, 2 isoenzymes: A and B Rhodococcus opacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 0.75
-
 isoenzyme B, hydrolysis of sedoheptulose 1,6-diphosphate Rhodococcus opacus
3.1.3.11 3.39
-
 isoenzyme B, hydrolysis of fructose 1,6-diphosphate Rhodococcus opacus
3.1.3.11 11.82
-
 isoenzyme A, hydrolysis of fructose 1,6-diphosphate Rhodococcus opacus
3.1.3.11 20.45
-
 isoenzyme B, hydrolysis of sedoheptulose 1,6-diphosphate Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O isoenzyme A is present when the organism is grown under autotrophic conditions but is not synthesized during heterotrophic growth. Isoenzyme B is detected in both autothrophically and heterotrophically grown organisms Rhodococcus opacus ?
-
 ?
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Rhodococcus opacus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 sedoheptulose 1,7-bisphosphate + H2O
-
 Rhodococcus opacus sedoheptulose 7-phosphate + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.2
-
 isoenzyme A, hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate Rhodococcus opacus
3.1.3.11 8.8 9 isoenzyme B, hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate Rhodococcus opacus