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Literature summary extracted from

  • Han, P.F.; Johnson, J.
    Fructose-1,6-bisphosphatase from turkey liver (1982), Methods Enzymol., 90, 334-340.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.11 immobilization on CNBr-activated Sepharose 4B, 57% loss of activity Meleagris gallopavo

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP sensitivity decreases greatly with increasing pH or temperature. Inhibition of Zn2+ and AMP is synergistic Meleagris gallopavo
3.1.3.11 Mg2+ inhibits above the optimal concentration of 1-1.5 mM Meleagris gallopavo
3.1.3.11 Mn2+ inhibits above the optimal concentration of 0.05-0.07 mM Meleagris gallopavo
3.1.3.11 Zn2+ pH 7.5, 0.0015 mM, 92% inhibition. Inhibition of Zn2+ and AMP is synergistic Meleagris gallopavo

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 K+ activates Meleagris gallopavo
3.1.3.11 Mg2+ optimal concentration at pH 7.5: 1-1.5 mM Meleagris gallopavo
3.1.3.11 Mg2+ divalent cation required. Mn2+ or Mg2+ Meleagris gallopavo
3.1.3.11 Mn2+ divalent cation required. Mn2+ or Mg2+ Meleagris gallopavo
3.1.3.11 Mn2+ optimal concentration at pH 7.5: 0.05-0.07 mM Meleagris gallopavo
3.1.3.11 Tl+ activates Meleagris gallopavo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 36000
-
 4 * 36000, SDS-PAGE Meleagris gallopavo
3.1.3.11 144000
-
 sucrose density gradient centrifugation Meleagris gallopavo

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Meleagris gallopavo
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Meleagris gallopavo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 liver
-
 Meleagris gallopavo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 25.3
-
-
 Meleagris gallopavo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Meleagris gallopavo D-fructose 6-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 tetramer 4 * 36000, SDS-PAGE Meleagris gallopavo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 7.4 7.6 in presence of chelating agents Meleagris gallopavo
3.1.3.11 8.4 8.5 without chelating agents Meleagris gallopavo