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Literature summary extracted from
- Characteristics of fructose-1,6-bisphosphatase from the methanol-ultilizing yeast Hansenula polymorpha (1982), J. Gen. Microbiol., 128, 2313-2317.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | EDTA | stimulates | Ogataea angusta |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | AMP | - |
Ogataea angusta | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.11 | 0.022 | - |
fructose 1,6-diphosphate | enzyme from ethanol-grown cells | Ogataea angusta | |
| 3.1.3.11 | 0.029 | - |
fructose 1,6-diphosphate | enzyme from methanol-grown cells | Ogataea angusta | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | Mg2+ | required, enzyme from methanol-grown cells | Ogataea angusta | |
| 3.1.3.11 | Mn2+ | can substitute for Mg2+ with 50% efficiency, enzyme from methanol-grown cells | Ogataea angusta | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.11 | Ogataea angusta | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.11 | partial | Ogataea angusta |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.11 | additional information | the enzyme purified from ethanol-grown cells resembles that from methanol-grown cells in all properties investigated | Ogataea angusta | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.11 | -20°C, half-life: 1 week, enzyme from methanol-grown cells | Ogataea angusta |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | - |
Ogataea angusta | D-fructose 6-phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 30 | - |
half-life: 1 h, enzyme from methanol-grown cells | Ogataea angusta |
| 3.1.3.11 | 37 | - |
half-life: 5 min, enzyme from methanol-grown cells | Ogataea angusta |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 8.5 | - |
enzyme from methanol-grown cells | Ogataea angusta |
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Release 2023.1 (January 2023)
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