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Literature summary extracted from

  • Friedberg, E.C.; Ganesan, A.K.; Seawell, P.C.
    Purification and properties of a pyrimidine dimer-specific endonuclease from E. coli infected with bacteriophage T4 (1980), Methods Enzymol., 65, 191-201.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.25.1 p-chloromercuribenzoate 70-80% inhibition in presence of 0.0004 M Tequatrovirus T4
3.1.25.1 p-chloromercuriphenylsulfonic acid 20-25% inhibition in presence of 0.0001 M Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.25.1 Mg2+ slight stimulation Tequatrovirus T4
3.1.25.1 Mn2+ slight stimulation Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.25.1 18000
-
PAGE, gel filtration Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Tequatrovirus T4
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.25.1 100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin Tequatrovirus T4

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.25.1 infected cell T4 infected Tequatrovirus T4
-

Storage Stability

EC Number Storage Stability Organism
3.1.25.1 -12°C, in 12% glycerol, inactivates the enzyme Tequatrovirus T4
3.1.25.1 4°C, in 50 mM Tris-HCl, pH 7.5, containing 10% ethylene glycol or 3% polyethylene glycol, storage for several months Tequatrovirus T4
3.1.25.1 freezing, leads to inactivation Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 ColE1 DNA + H2O UV-irradiated, superhelical Tequatrovirus T4 oligonucleotides
-
?
3.1.25.1 DNA + H2O
-
Tequatrovirus T4 ?
-
?
3.1.25.1 E. coli DNA + H2O single-stranded DNA Tequatrovirus T4 oligonucleotides
-
?
3.1.25.1 E. coli DNA + H2O UV-irradiated dsDNA Tequatrovirus T4 oligonucleotides
-
?
3.1.25.1 glycosylated DNA + H2O UV-irradiated, cleaves also nonglycosylated DNA Tequatrovirus T4 oligonucleotides
-
?
3.1.25.1 additional information not: 5-thyminyl-5,6-dihydrothymine-containing DNA Tequatrovirus T4 ?
-
?
3.1.25.1 additional information not: DNA exposed to ionizing radiation, methyl methane sulfonate, nitrogen mustard, mitomycin C, 4-nitroquinoline-1-oxide Tequatrovirus T4 ?
-
?
3.1.25.1 PBS1 DNA UV irradiated, contains uracil instead of thymine Tequatrovirus T4 oligonucleotides
-
?
3.1.25.1 SPO1 DNA UV irradiated, contains 5-hydroxymethyluracil instead of thymine Tequatrovirus T4 oligonucleotides
-
?
3.1.25.1 superhelical DNA + H2O UV-irradiated, cleaves also linear DNA Tequatrovirus T4 oligonucleotides
-
?