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Literature summary extracted from

  • Ishida, M.; Kanamori, Y.; Hori, N.; Inaoka, T.; Ohtsuka, E.
    In vitro and in vivo activities of T4 endonuclease V mutants altered in the C-terminal aromatic region (1990), Biochemistry, 29, 3817-3821.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.17 pEVC127, cloned and expressed in Escherichia coli HB101, synthetic gene constructed by ligation of oligonucleotide fragments expressed efficiently in Escherichia coli Tequatrovirus T4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.17
-
 Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.25.1 DELTAC127 C127-truncated, total loss of activity Tequatrovirus T4
3.1.25.1 K130L almost same activity as wild-type Tequatrovirus T4
3.1.25.1 T127M almost same activity as wild-type, Km value in the same order as that of wild-type Tequatrovirus T4
3.1.25.1 W128A total loss of activity Tequatrovirus T4
3.1.25.1 W128S only slight activity remains Tequatrovirus T4
3.1.25.1 Y129A total loss of activity Tequatrovirus T4
3.1.25.1 Y131A only slight activity remains Tequatrovirus T4
3.1.25.1 Y132A only slight activity remains Tequatrovirus T4

General Stability

EC Number General Stability Organism
3.1.25.1 addition of 8 M urea leads to denaturation Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.25.1 0.000015
-
 DNA 14mer containing a TT-dimer WT-enzyme and T127M Tequatrovirus T4
3.2.2.17 0.000015
-
 DNA wild-type Tequatrovirus T4
3.2.2.17 0.000015
-
 DNA mutant Met-127 Tequatrovirus T4
3.2.2.17 0.0001
-
 DNA mutant T127M, same Km value as the wild-type Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Tequatrovirus T4
-
-
-
3.2.2.17 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.25.1 T127M Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 5'-side of dimers Tequatrovirus T4
a
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.25.1
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.25.1 DNA 14mer containing a TT-dimer + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.25.1 oligodeoxyribonucleotides 14mer containing a TT-dimer, duplex Tequatrovirus T4 oligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.17 PD-glycosylase
-
 Tequatrovirus T4
3.2.2.17 thymine photodimer repair enzyme
-
 Tequatrovirus T4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.25.1 0.00833
-
 DNA 14mer containing a TT-dimer T127M Tequatrovirus T4
3.1.25.1 0.0183
-
 DNA 14mer containing a TT-dimer WT-enzyme Tequatrovirus T4