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Literature summary extracted from

  • Stump, D.G.; Lloyd, R.S.
    Site-directed mutagenesis of the T4 endonuclease V gene: role of tyrosine-129 and -131 in pyrimidine dimer-specific binding (1988), Biochemistry, 27, 1839-1843.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.25.1 expressed in Escherichia coli Tequatrovirus T4
3.2.2.17 cloned into a variety of plasmids, express endonuclease V activity in repair-deficient Escherichia coli Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.25.1 Y129N dimer-specific binding and nicking abolished, apurinic-specific nicking abolished Tequatrovirus T4
3.1.25.1 Y131N in combination with Y129N, dimer-specific binding and nicking abolished, ability for apurinic-specific nicking retained Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Tequatrovirus T4
-
-
-
3.2.2.17 Tequatrovirus T4
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.25.1 native DNA + H2O E. coli DNA in vivo Tequatrovirus T4 oligonucleotides
-
 ?
3.1.25.1 pBR322 DNA + H2O UV-irradiated Tequatrovirus T4 oligonucleotides
-
 ?
3.2.2.17 DNA + H2O double stranded DNA containing pyrimidine dimers Tequatrovirus T4 thymine + ?
-
 ?