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Literature summary extracted from

  • Recinos , A.; Lloyd, R.S.
    Site-directed mutagenesis of the T4 endonuclease V gene: role of lysine-130 (1988), Biochemistry, 27, 1832-1838.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.25.1 expressed in Escherichia coli Tequatrovirus T4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.17
-
 Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.25.1 K130G diminished capacity to make complete incisions, significantly decreased intracellular accumulation Tequatrovirus T4
3.1.25.1 K130G low dimer-specific binding activity, AP-endonuclease function several times as efficient as that of the wild-type Tequatrovirus T4
3.1.25.1 K130H diminished capacity to make complete incisions, slight decreased intracellular accumulation Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Tequatrovirus T4
-
 ?
-
 ?
3.2.2.17 DNA + H2O Tequatrovirus T4 removal of UV-induced pyrimidine dimer photoproducts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Tequatrovirus T4
-
-
-
3.2.2.17 Tequatrovirus T4
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 3'-side of the apyrimidinic site Tequatrovirus T4
a
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a

Storage Stability

EC Number Storage Stability Organism
3.1.25.1 4°C, stock enzyme-containing cell extracts Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.25.1 E. coli DNA + H2O
-
 Tequatrovirus T4 oligonucleotides
-
 ?
3.1.25.1 native DNA + H2O E. coli DNA in vivo Tequatrovirus T4 oligonucleotides
-
 ?
3.1.25.1 pBR322 DNA + H2O UV-irradiated Tequatrovirus T4 oligonucleotides
-
 ?
3.2.2.17 DNA + H2O removal of UV-induced pyrimidine dimer photoproducts Tequatrovirus T4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.17 denV
-
 Tequatrovirus T4
3.2.2.17 endonuclease V
-
 Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.17 6.5
-
 2 distinct domains within the single polypeptide, the 2 activities possess different optima for pH and salt concentration and different degrees of thermolability Tequatrovirus T4