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Literature summary extracted from

  • Minton, K.; Durphy, M.; Taylor, R.; Friedberg, E.C.
    The ultraviolet endonuclease of bacteriophage T4. Further characterization (1975), J. Biol. Chem., 250, 2823-2829.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.25.1 additional information endonuclease activity not affected by: -SH-group inhibitors, caffeine, tRNA Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.25.1 17700
-
 SDS-PAGE Tequatrovirus T4
3.1.25.1 18000
-
 gel filtration Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.25.1
-
 Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 5'-side of dimers Tequatrovirus T4
a

Storage Stability

EC Number Storage Stability Organism
3.1.25.1 4°C, in 10 mM Tris-HCl buffer, pH 8.0, with 3% polyethylene glycol and 0.1 mM EDTA Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.25.1 E. coli DNA + H2O single-stranded DNA Tequatrovirus T4 oligonucleotides
-
 ?
3.1.25.1 E. coli DNA + H2O UV-irradiated dsDNA Tequatrovirus T4 oligonucleotides
-
 ?
3.1.25.1 M-13 DNA + H2O UV-irradiated, single-stranded DNA Tequatrovirus T4 oligonucleotides
-
 ?
3.1.25.1 T7 DNA + H2O UV-irradiated Tequatrovirus T4 oligonucleotides
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.25.1 7 8 pH-optimum of endonuclease activity Tequatrovirus T4