EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.25.1 | NaCl | inhibits endonuclease activity | Tequatrovirus T4 | |
3.2.2.17 | NaCl | nicking activity rather inhibited | Tequatrovirus T4 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.25.1 | NaCl | activating | Tequatrovirus T4 | |
3.1.25.1 | NaCl | optimum for the glycosylase: 25-100 mM | Tequatrovirus T4 | |
3.2.2.17 | NaCl | activity to produce alkali-labile sites stimulated by 25-100 mM | Tequatrovirus T4 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.25.1 | 16000 | - |
SDS-PAGE | Tequatrovirus T4 |
3.2.2.17 | 16000 | - |
- |
Tequatrovirus T4 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.25.1 | DNA + H2O | Tequatrovirus T4 | - |
? | - |
? | |
3.2.2.17 | additional information | Tequatrovirus T4 | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.25.1 | Tequatrovirus T4 | - |
- |
- |
3.2.2.17 | Tequatrovirus T4 | - |
T4 D, T4v1, T4uvs-5, T4uvs-13, host Escherichia coli B, Escherichia coli 1100, Escherichia coli Cr63 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.25.1 | 4100fold, to homogeneity | Tequatrovirus T4 |
3.2.2.17 | - |
Tequatrovirus T4 |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.25.1 | endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease | Tequatrovirus T4 |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.25.1 | infected cell | T4 infected | Tequatrovirus T4 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.25.1 | additional information | - |
- |
Tequatrovirus T4 |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.25.1 | -20°C, addition of an equal value of glycerol | Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.25.1 | DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
3.1.25.1 | poly(dA)-poly(dT) + H2O | UV-irradiated | Tequatrovirus T4 | oligonucleotides | length is less than 15 nucleotides | ? | |
3.2.2.17 | additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Tequatrovirus T4 | ? | - |
? | |
3.2.2.17 | poly(dAdT) + H2O | - |
Tequatrovirus T4 | oligonucleotides | acid-soluble oligonucleotides, length less than 15 nucleotides | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.17 | monomer | 1 * 17500-18000, single polypeptide chain | Tequatrovirus T4 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.25.1 | 6 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |
3.1.25.1 | 6 | 8.5 | pH-optimum of glycosylase activity | Tequatrovirus T4 |
3.1.25.1 | 6.5 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |
3.2.2.17 | 6.5 | - |
- |
Tequatrovirus T4 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.17 | 6 | 8.5 | - |
Tequatrovirus T4 |