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Literature summary extracted from

  • Nakabeppu, Y.; Yamashita, K.; Sekiguchi, M.
    Purification and characterization of normal and mutant forms of T4 endonuclease V (1982), J. Biol. Chem., 257, 2556-2562.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.25.1 NaCl inhibits endonuclease activity Tequatrovirus T4
3.2.2.17 NaCl nicking activity rather inhibited Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.25.1 NaCl activating Tequatrovirus T4
3.1.25.1 NaCl optimum for the glycosylase: 25-100 mM Tequatrovirus T4
3.2.2.17 NaCl activity to produce alkali-labile sites stimulated by 25-100 mM Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.25.1 16000
-
 SDS-PAGE Tequatrovirus T4
3.2.2.17 16000
-
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Tequatrovirus T4
-
 ?
-
 ?
3.2.2.17 additional information Tequatrovirus T4 only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Tequatrovirus T4
-
-
-
3.2.2.17 Tequatrovirus T4
-
 T4 D, T4v1, T4uvs-5, T4uvs-13, host Escherichia coli B, Escherichia coli 1100, Escherichia coli Cr63
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.25.1 4100fold, to homogeneity Tequatrovirus T4
3.2.2.17
-
 Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.25.1 endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.25.1 infected cell T4 infected Tequatrovirus T4
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.25.1 additional information
-
-
 Tequatrovirus T4

Storage Stability

EC Number Storage Stability Organism
3.1.25.1 -20°C, addition of an equal value of glycerol Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.25.1 poly(dA)-poly(dT) + H2O UV-irradiated Tequatrovirus T4 oligonucleotides length is less than 15 nucleotides ?
3.2.2.17 additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Tequatrovirus T4 ?
-
 ?
3.2.2.17 poly(dAdT) + H2O
-
 Tequatrovirus T4 oligonucleotides acid-soluble oligonucleotides, length less than 15 nucleotides ?

Subunits

EC Number Subunits Comment Organism
3.2.2.17 monomer 1 * 17500-18000, single polypeptide chain Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.25.1 6
-
 pH-optimum of endonuclease activity Tequatrovirus T4
3.1.25.1 6 8.5 pH-optimum of glycosylase activity Tequatrovirus T4
3.1.25.1 6.5
-
 pH-optimum of endonuclease activity Tequatrovirus T4
3.2.2.17 6.5
-
-
 Tequatrovirus T4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.17 6 8.5
-
 Tequatrovirus T4