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Literature summary extracted from

  • Takimoto, A.; Yagi, S.; Mitsushima, K.
    High-level expression, purification, and some properties of a recombinant cephalosporin-C deacetylase (1999), J. Biosci. Bioeng., 87, 456-462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.41 expression in Escherichia coli Bacillus subtilis
3.1.1.41 the most efficient expression plasmid is pCAH431, the expression and purification system has made the industrial production of the enzyme possible Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 7.4
-
 7-aminocephalosporanic acid recombinant enzyme, 30°C Bacillus subtilis
3.1.1.41 9.2
-
 cephaloglycin recombinant enzyme Bacillus subtilis
3.1.1.41 11.7
-
 cephalotin recombinant enzyme Bacillus subtilis
3.1.1.41 16.7
-
 cephapirin recombinant enzyme Bacillus subtilis
3.1.1.41 34.5
-
 cephalosporin C recombinant enzyme Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 35000
-
 8 * 35000, strain SHS 0133, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis
-
-
-
3.1.1.41 Bacillus subtilis SHS 0133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41 recombinant enzyme, the expression and purification system has made the industrial production of the enzyme possible Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.41 210
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
3.1.1.41 4°C, stable for 1 year Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis SHS 0133 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 cephaloglycin + H2O
-
 Bacillus subtilis acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.1.1.41 cephaloglycin + H2O
-
 Bacillus subtilis SHS 0133 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Bacillus subtilis deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis acetate + deacetylcephalothin
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis SHS 0133 acetate + deacetylcephalothin
-
 ?
3.1.1.41 cephapirin + H2O
-
 Bacillus subtilis acetate + deacetylcephapirin
-
 ?
3.1.1.41 cephapirin + H2O
-
 Bacillus subtilis SHS 0133 acetate + deacetylcephapirin
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Bacillus subtilis SHS 0133 4-nitrophenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.41 octamer 8 * 35000, strain SHS 0133, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.41 CAH
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 55
-
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 8 8.5
-
 Bacillus subtilis