Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takimoto, A.; Mitsushima, K.; Yagi, S.; Sonoyama, T.
    Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis (1994), J. Ferment. Bioeng., 77, 17-22.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 acetate competitive; weak Bacillus subtilis
3.1.1.41 Cu2+
-
 Bacillus subtilis
3.1.1.41 deacetyl-7-aminocephalosporanic acid weak noncompetitive Bacillus subtilis
3.1.1.41 DFP
-
 Bacillus subtilis
3.1.1.41 additional information the weak product inhibitors do not prevent the completion of the deacetylation Bacillus subtilis
3.1.1.41 N-tosyl-L-Lys chloromethyl ketone
-
 Bacillus subtilis
3.1.1.41 Zn2+
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 1
-
 p-nitrophenyl acetate
-
 Bacillus subtilis
3.1.1.41 6.5
-
 7-aminocephalosporanic acid strain ATCC6633 Bacillus subtilis
3.1.1.41 7.9
-
 7-aminocephalosporanic acid strain SHS 0133, FERM BP-2755 Bacillus subtilis
3.1.1.41 11.1
-
 cephalotin
-
 Bacillus subtilis
3.1.1.41 11.5
-
 cephaloglycin
-
 Bacillus subtilis
3.1.1.41 13.9
-
 cephapirin
-
 Bacillus subtilis
3.1.1.41 24
-
 cephalosporin C
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 35000
-
 8 * 35000, strain SHS 0133, SDS-PAGE Bacillus subtilis
3.1.1.41 150000
-
 strain ATCC6633 Bacillus subtilis
3.1.1.41 280000
-
 strain SHS 0133, gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis
-
-
-
3.1.1.41 Bacillus subtilis
-
 strain ATCC6633
-
3.1.1.41 Bacillus subtilis ATCC 6633
-
 strain ATCC6633
-
3.1.1.41 Bacillus subtilis SHS 0133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41 strain SHS 0133, FERM BP-2755 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.41 187.4
-
 hydrolysis of p-nitrophenyl acetate Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis ATCC 6633 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis SHS 0133 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 cephaloglycin + H2O
-
 Bacillus subtilis acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.1.1.41 cephaloglycin + H2O
-
 Bacillus subtilis ATCC 6633 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.1.1.41 cephaloglycin + H2O
-
 Bacillus subtilis SHS 0133 acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Bacillus subtilis deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis acetate + deacetylcephalothin
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis ATCC 6633 acetate + deacetylcephalothin
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis SHS 0133 acetate + deacetylcephalothin
-
 ?
3.1.1.41 cephapirin + H2O
-
 Bacillus subtilis acetate + deacetylcephapirin
-
 ?
3.1.1.41 cephapirin + H2O
-
 Bacillus subtilis ATCC 6633 acetate + deacetylcephapirin
-
 ?
3.1.1.41 cephapirin + H2O
-
 Bacillus subtilis SHS 0133 acetate + deacetylcephapirin
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Bacillus subtilis ATCC 6633 4-nitrophenol + acetate
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Bacillus subtilis SHS 0133 4-nitrophenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.41 octamer 8 * 35000, strain SHS 0133, SDS-PAGE Bacillus subtilis
3.1.1.41 tetramer strain ATCC6633 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 55
-
 strain SHS 0133, FERM BP-2755 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 30
-
 pH 7.0, stable for at least 1 month Bacillus subtilis
3.1.1.41 60
-
 pH 7.0, 30 min, stable up to Bacillus subtilis
3.1.1.41 65
-
 pH 7.0, 30 min, 70% loss of activity Bacillus subtilis
3.1.1.41 70
-
 pH 7.0, 30 min, complete loss of activity Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.41 55
-
 cephalothin
-
 Bacillus subtilis
3.1.1.41 80
-
 cephapirin
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 8
-
 strain ATCC6633 Bacillus subtilis
3.1.1.41 8 8.5
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.41 7 10 37°C, 30 min, stable Bacillus subtilis