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Literature summary extracted from

  • Sakai, Y.; Abe, T.; Aoki, M.; Ohabayashi, Y.; Yamamoto, K.; Tani, Y.; Kato, N.
    Purification and properties of cephalosporin-C deacetylase from the yeast, Rhodotorula glutinis 38B1, useful for bioconversion of 7-aminocephalosporanic acid derivatives (1998), J. Ferment. Bioeng., 85, 53-57.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 (p-amino-phenyl)methanesulfonyl fluoride slight inhibition Rhodotorula glutinis
3.1.1.41 5,5'-dithiobis(2-nitrobenzoate)
-
 Rhodotorula glutinis
3.1.1.41 Cu2+ CuCl2 Rhodotorula glutinis
3.1.1.41 eserine sulfate slight inhibition Rhodotorula glutinis
3.1.1.41 Hg2+ HgCl2 Rhodotorula glutinis
3.1.1.41 PbCl2
-
 Rhodotorula glutinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 1.11
-
 benzyloxycarbonyl-7-aminocephalosporanic acid
-
 Rhodotorula glutinis
3.1.1.41 1.43
-
 7-aminocephalosporanic acid
-
 Rhodotorula glutinis
3.1.1.41 2.03
-
 monochloroacetyl-7-aminocephalosporanic acid
-
 Rhodotorula glutinis
3.1.1.41 8.39
-
 cephalosporin C
-
 Rhodotorula glutinis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.41 cell wall the majority of the enzyme is tightly bound to the cell wall Rhodotorula glutinis 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 82000
-
 2 * 82000, SDS-PAGE Rhodotorula glutinis
3.1.1.41 141000
-
 gel filtration Rhodotorula glutinis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Rhodotorula glutinis
-
-
-
3.1.1.41 Rhodotorula glutinis 38B1
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.41 side-chain modification N-linked sugar content is about 21% by weight Rhodotorula glutinis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41
-
 Rhodotorula glutinis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.41 342
-
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Rhodotorula glutinis acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Rhodotorula glutinis 38B1 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 benzyloxycarbonyl-7-aminocephalosporanic acid + H2O
-
 Rhodotorula glutinis ?
-
 ?
3.1.1.41 benzyloxycarbonyl-7-aminocephalosporanic acid + H2O
-
 Rhodotorula glutinis 38B1 ?
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Rhodotorula glutinis deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Rhodotorula glutinis 38B1 deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 monochloroacetyl-7-aminocephalosporanic acid + H2O
-
 Rhodotorula glutinis acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 monochloroacetyl-7-aminocephalosporanic acid + H2O
-
 Rhodotorula glutinis 38B1 acetate + monochloroacetyl-deacetyl-7-aminocephalosporanic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.41 dimer 2 * 82000, SDS-PAGE Rhodotorula glutinis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 35
-
-
 Rhodotorula glutinis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.41 10 45 10°C: about 40% of maximal activity, 45°C: about 30% of maximal activity Rhodotorula glutinis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 30
-
 15 min, 60% loss of activity Rhodotorula glutinis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.41 534
-
 benzyloxycarbonyl-7-aminocephalosporanic acid
-
 Rhodotorula glutinis
3.1.1.41 576
-
 7-aminocephalosporanic acid
-
 Rhodotorula glutinis
3.1.1.41 768
-
 monochloroacetyl-7-aminocephalosporanic acid
-
 Rhodotorula glutinis
3.1.1.41 1370
-
 cephalosporin C
-
 Rhodotorula glutinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 5.5
-
-
 Rhodotorula glutinis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.41 4.2 7 pH 4.2: 30% of maximal activity, pH 7.0: about 35% of maximal activity Rhodotorula glutinis