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Literature summary extracted from

  • Abbott, B.J.; Fukuda, D.S.
    Cephalosporin acetylesterase (Bacillus subtilis) (1978), Methods Enzymol., 43, 731-734.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 acetate competitive Bacillus subtilis
3.1.1.41 deacetyl-7-aminocephalosporanic acid competitive Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 0.46
-
 Triacetin
-
 Bacillus subtilis
3.1.1.41 2.8
-
 7-aminocephalosporanic acid
-
 Bacillus subtilis
3.1.1.41 8.3
-
 cephalothin
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis
-
-
-
3.1.1.41 Bacillus subtilis WRRL-B-558
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.41 25°C, pH 7, unbuffered solution, 3 weeks, little or no loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 7-aminocephalosporanic acid + H2O
-
 Bacillus subtilis WRRL-B-558 acetate + deacetyl-7-aminocephalosporanic acid
-
 ?
3.1.1.41 alpha-naphthyl acetate + H2O
-
 Bacillus subtilis 1-naphthol + acetate
-
 ?
3.1.1.41 alpha-naphthyl acetate + H2O
-
 Bacillus subtilis WRRL-B-558 1-naphthol + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Bacillus subtilis deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis acetate + deacetylcephalothin
-
 ?
3.1.1.41 cephalothin + H2O
-
 Bacillus subtilis WRRL-B-558 acetate + deacetylcephalothin
-
 ?
3.1.1.41 glucose pentaacetate + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.41 glucose pentaacetate + H2O
-
 Bacillus subtilis WRRL-B-558 ?
-
 ?
3.1.1.41 monoacetin + H2O
-
 Bacillus subtilis acetate + glycerol
-
 ?
3.1.1.41 triacetin + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.41 triacetin + H2O
-
 Bacillus subtilis WRRL-B-558 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 40 50
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 80
-
 complete loss of activity after 5 min, activity is retained after 10 min in presence of 1.0 M phosphate buffer Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 7
-
-
 Bacillus subtilis