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Literature summary extracted from
- Physical properties and kinetic behavior of a cephalosporin acetylesterase produced by Bacillus subtilis (1975), Appl. Microbiol., 30, 413-419.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.41 | acetate | - |
Bacillus subtilis |  |
| 3.1.1.41 | deacetyl-7-aminocephalosporanic acid | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.41 | 2.8 | - |
7-aminocephalosporanic acid | - |
Bacillus subtilis | |
| 3.1.1.41 | 8.3 | - |
cephalothin | - |
Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.41 | 190000 | - |
gel filtration | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.41 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.41 | partial | Bacillus subtilis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.41 | 25°C, 1 month, more than 80% of the activity is retained | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.41 | 7-aminocephalosporanic acid + H2O | - |
Bacillus subtilis | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| 3.1.1.41 | cephalothin + H2O | - |
Bacillus subtilis | acetate + deacetylcephalothin | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.41 | 40 | 50 | - |
Bacillus subtilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.41 | 20 | 60 | 20°C: about 40% of maximal activity, 60°C: about 10% of maximal activity | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.41 | 25 | - |
1 month, less than 20% loss of activity | Bacillus subtilis |
| 3.1.1.41 | 70 | - |
salt-free enzyme, 5 min, 50% loss of activity | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.41 | 7 | - |
- |
Bacillus subtilis |
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Release 2023.1 (January 2023)
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