Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luehr, C.A.; Schuster, S.M.
    Purification and characterization of beef pancreatic asparagine synthetase (1985), Arch. Biochem. Biophys., 237, 335-346.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 Cl- inhibition of the ammonia-dependent reaction, competitive with respect to ammonia, with negative cooperativity. Stimulation of the Gln-dependent and glutaminase reaction Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.6
-
 Asp
-
 Bos taurus
6.3.5.4 0.7
-
 ATP
-
 Bos taurus
6.3.5.4 1.4
-
 Gln
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Ca2+ at 16.7 mM CaCl2 31% of the activation relative to MgCl2 Bos taurus
6.3.5.4 Cl- stimulation of the Gln-dependent reaction and glutaminase reaction. Inhibition of the NH4+-dependent reaction, competitive with respect to NH4+, with negative cooperativity Bos taurus
6.3.5.4 Co2+ at 16.7 mM CoCl2 31% of the activation relative to MgCl2 Bos taurus
6.3.5.4 FeCl2 at 16.7 mM FeCl2 39% of the activation relative to MgCl2 Bos taurus
6.3.5.4 Mg2+ 16.7 mM MgCl2, activates Bos taurus
6.3.5.4 Mn2+ at 16.7 mM MnCl2 14% of the activation relative to MgCl2 Bos taurus
6.3.5.4 Ni2+ at 16.7 mM NiCl2 17% of the activation relative to MgCl2 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.4 60000
-
 x * 60000, SDS-PAGE Bos taurus
6.3.5.4 110000 120000 HPLC gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.4 0.17
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Bos taurus AMP + diphosphate + Asn + Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.4 ? x * 60000, SDS-PAGE Bos taurus