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Literature summary extracted from

  • Schendel, F.J.; Mueller, E.; Stubbe, J.
    Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization (1989), Biochemistry, 28, 2459-2471.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.3
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.3 albizziin
-
 Escherichia coli
6.3.5.3 Glu
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.3 0.03
-
 L-Gln 5'-phosphoribosylformylglycinamidine Escherichia coli
6.3.5.3 0.0468
-
 (-)carbocyclic-5'-phosphoribosylformylglycinamidine
-
 Escherichia coli
6.3.5.3 1
-
 NH4+
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.3 K+ maximal activity at 10 mM K+ Escherichia coli
6.3.5.3 Mg2+ maximal activation at 20 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.3 141418
-
 x * 141418, calculation from nucleotide sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.3 2.15
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Escherichia coli ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O
-
 Escherichia coli ADP + phosphate + 5'-phosphoribosylformylglycinamidine
-
 ?
6.3.5.3 ATP + carbocyclic-5'-phosphoribosylformylglycinamide + L-Gln + H2O (+/-)carbocyclic-5'-phosphoribosylformylglycinamide and (-)5'-phosphoribosylformylglycinamide at 10% of the activity relative to 5'-phosphoribosylformylglycinamide Escherichia coli ADP + phosphate + carbocyclic-5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.3 ? x * 141418, calculation from nucleotide sequence Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.3 0.05
-
 NH4+
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.3 7.2
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.3 6.2 8 about 50% of maximal activity at pH 6.2 and 8.0 Escherichia coli