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Literature summary extracted from

  • Buchanan, J.M.; Ohnoki, S.; Hong, B.S.
    2-Formamido-N-ribosylacetamide 5'-phosphate:L-glutamine amido-ligase (adenosine diphosphate) (1978), Methods Enzymol., 51, 193-201.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.3 6-diazo-5-oxo-L-norleucine competitive analogue of Gln binds irreversibly at the Gln active site Gallus gallus
6.3.5.3 albizziin competitive analogue of Gln binds irreversibly at the Gln active site Gallus gallus
6.3.5.3 Cyanate
-
Gallus gallus
6.3.5.3 hydroxylamine
-
Gallus gallus
6.3.5.3 iodoacetamide
-
Gallus gallus
6.3.5.3 iodoacetate
-
Gallus gallus
6.3.5.3 O-diazoacetyl-L-Ser i.e. L-azaserine, competitive analogue of Gln binds irreversibly at the Gln active site Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.3 0.1
-
5'-phosphoribosylformylglycinamide
-
Gallus gallus
6.3.5.3 0.2
-
Gln
-
Gallus gallus
6.3.5.3 0.4
-
ATP
-
Gallus gallus
6.3.5.3 80 100 NH4+
-
Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.3 Mg2+ required Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.3 133000 135000
-
Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 133000 135000
-
Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.3 Gallus gallus
-
-
-
6.3.5.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.3
-
Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.5.3 ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate Gln adds first to the enzyme with ATP and 5'-phosphoribosylformylglycinamide adding randomly subsequently Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.3 liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.3 additional information
-
-
Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
Gallus gallus ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O
-
Gallus gallus ADP + phosphate + 5'-phosphoribosylformylglycinamidine
-
?
6.3.5.3 ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O ATP in form of MgATP2- Gallus gallus ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
?
6.3.5.3 additional information glutamyl derivatives, e.g. gamma-glutamylhydroxamate, gamma-glutamyl hydrazine, gamma-glutamyl esters, and gamma-glutamyl thioesters are hydrolyzed with strict dependence on Mg2+, ATP, and 5'-phosphoribosylformylglycinamide Gallus gallus ?
-
?