BRENDA - Enzyme Database

The role of transacylases in the metabolism of arachidonate and platelet activating factor

Snyder, F.; Lee, T.C.; Blank, M.L.; Prog. Lipid Res. 31, 65-86 (1992) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.148
Zn2+
strong
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.148
0.0015
-
1-hexadecyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
2.3.1.148
0.0038
-
1-acyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
2.3.1.148
0.017
-
1-palmitoyl-2-lyso-glycerophosphocholine
-
Rattus norvegicus
2.3.1.148
0.076
-
lyso-phosphatidylserine
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.148
microsome
-
Rattus norvegicus
-
-
2.3.1.148
microsome
-
Oryctolagus cuniculus
-
-
2.3.1.148
microsome
-
Canis lupus familiaris
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.148
Canis lupus familiaris
-
-
-
2.3.1.148
Homo sapiens
-
-
-
2.3.1.148
Mus musculus
-
-
-
2.3.1.148
Oryctolagus cuniculus
-
-
-
2.3.1.148
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.148
alveolar macrophage
-
Oryctolagus cuniculus
-
2.3.1.148
heart
-
Canis lupus familiaris
-
2.3.1.148
liver
-
Rattus norvegicus
-
2.3.1.148
liver
-
Oryctolagus cuniculus
-
2.3.1.148
lung
-
Rattus norvegicus
-
2.3.1.148
lymphocyte
-
Mus musculus
-
2.3.1.148
macrophage
-
Mus musculus
-
2.3.1.148
platelet
-
Homo sapiens
-
2.3.1.148
platelet
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.148
1-Acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
transfer of arachidonate occurs through a bidirectional movement
16278
Mus musculus
1-Acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
-
-
?
2.3.1.148
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + lyso-phosphatidylcholine
-
16278
Mus musculus
1-acyl-2-lyso-sn-glycero-3-phosphoinositol + ?
-
-
-
?
2.3.1.148
1-Acyl-2-arachidonoyl-sn-glycero-3-phosphophoinositol + lyso-phosphatidylethanolamine
unidirectional transfer process of arachidonate
16278
Mus musculus
1-Acyl-2-lyso-sn-glycero-3-phosphoinositol + arachidonoylphosphatidylethanolamine
-
-
-
?
2.3.1.148
1-acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Oryctolagus cuniculus
1-acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
2.3.1.148
1-Hexadecyl-2-lyso-glycerophosphocholine + phospholipid
-
16278
Oryctolagus cuniculus
?
-
-
-
?
2.3.1.148
1-palmitoyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Rattus norvegicus
1-palmitoyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
2.3.1.148
lyso-Pasmenylethanolamine + phospholipid
-
16278
Homo sapiens
?
-
-
-
?
2.3.1.148
lyso-Phosphatidylethanolamine + phospholipid
-
16278
Homo sapiens
Phosphatidylethanolamine + lyso-phospholipid
-
-
-
?
2.3.1.148
lyso-Phosphatidylinositol + phospholipid
-
16278
Homo sapiens
Phosphatidylinositol + lyso-phospholipid
-
-
-
?
2.3.1.148
lyso-Phosphatidylserine + phospholipid
-
16278
Homo sapiens
Phosphatidylserine + lyso-phospholipid
-
-
-
?
2.3.1.148
additional information
arachidonate and linoleate at the sn-2 position of phosphatidylcholine can be transferred to lyso-phosphatidylethanolamine. The transfer of 16:0, 18:0, and 18:1 acyl moieties at the sn-2-position of phosphatidylcholine is negligible
16278
Rattus norvegicus
?
-
-
-
?
2.3.1.148
additional information
more efficient transfer of arachidonate from phosphatidylcholine to the ethanolamine-containing phospholipids than from phosphatidylinositol
16278
Homo sapiens
?
-
-
-
?
2.3.1.148
Phosphatidylcholine + lyso-phosphatidylethanolamine
-
16278
Mus musculus
lyso-Phosphatidylcholine + phosphatidylethanolamine
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.148
4.5
-
bimodal pH-optimum, at pH 4.5 and at pH 7.5. The activity is 4-5times higher at pH 4.5 than at pH 7.5
Canis lupus familiaris
2.3.1.148
7.5
-
-
Rattus norvegicus
2.3.1.148
7.5
-
bimodal pH-optimum, at pH 7.5 and at pH 4.5
Canis lupus familiaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.148
CoA
-
Canis lupus familiaris
2.3.1.148
CoA
required
Mus musculus
2.3.1.148
CoA
required
Homo sapiens
2.3.1.148
CoA
required
Rattus norvegicus
2.3.1.148
CoA
required
Oryctolagus cuniculus
2.3.1.148
CoA
Km: 0.0015 mM, lung enzyme, with lyso-phosphatidylethanolamine as substrate
Rattus norvegicus
2.3.1.148
CoA
Km: 0.014 mM, liver enzyme, with 2-lyso-sn-phosphatidylinositol as substrate
Rattus norvegicus
2.3.1.148
CoA
Km: 0.0014 mM
Homo sapiens
2.3.1.148
CoA
Km: 0.0014 mM
Oryctolagus cuniculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.3.1.148
CoA
-
Canis lupus familiaris
2.3.1.148
CoA
required
Mus musculus
2.3.1.148
CoA
required
Homo sapiens
2.3.1.148
CoA
required
Rattus norvegicus
2.3.1.148
CoA
required
Oryctolagus cuniculus
2.3.1.148
CoA
Km: 0.0015 mM, lung enzyme, with lyso-phosphatidylethanolamine as substrate
Rattus norvegicus
2.3.1.148
CoA
Km: 0.014 mM, liver enzyme, with 2-lyso-sn-phosphatidylinositol as substrate
Rattus norvegicus
2.3.1.148
CoA
Km: 0.0014 mM
Homo sapiens
2.3.1.148
CoA
Km: 0.0014 mM
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.148
Zn2+
strong
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.148
0.0015
-
1-hexadecyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
2.3.1.148
0.0038
-
1-acyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
2.3.1.148
0.017
-
1-palmitoyl-2-lyso-glycerophosphocholine
-
Rattus norvegicus
2.3.1.148
0.076
-
lyso-phosphatidylserine
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.148
microsome
-
Rattus norvegicus
-
-
2.3.1.148
microsome
-
Oryctolagus cuniculus
-
-
2.3.1.148
microsome
-
Canis lupus familiaris
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.148
alveolar macrophage
-
Oryctolagus cuniculus
-
2.3.1.148
heart
-
Canis lupus familiaris
-
2.3.1.148
liver
-
Rattus norvegicus
-
2.3.1.148
liver
-
Oryctolagus cuniculus
-
2.3.1.148
lung
-
Rattus norvegicus
-
2.3.1.148
lymphocyte
-
Mus musculus
-
2.3.1.148
macrophage
-
Mus musculus
-
2.3.1.148
platelet
-
Homo sapiens
-
2.3.1.148
platelet
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.148
1-Acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
transfer of arachidonate occurs through a bidirectional movement
16278
Mus musculus
1-Acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
-
-
?
2.3.1.148
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + lyso-phosphatidylcholine
-
16278
Mus musculus
1-acyl-2-lyso-sn-glycero-3-phosphoinositol + ?
-
-
-
?
2.3.1.148
1-Acyl-2-arachidonoyl-sn-glycero-3-phosphophoinositol + lyso-phosphatidylethanolamine
unidirectional transfer process of arachidonate
16278
Mus musculus
1-Acyl-2-lyso-sn-glycero-3-phosphoinositol + arachidonoylphosphatidylethanolamine
-
-
-
?
2.3.1.148
1-acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Oryctolagus cuniculus
1-acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
2.3.1.148
1-Hexadecyl-2-lyso-glycerophosphocholine + phospholipid
-
16278
Oryctolagus cuniculus
?
-
-
-
?
2.3.1.148
1-palmitoyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Rattus norvegicus
1-palmitoyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
2.3.1.148
lyso-Pasmenylethanolamine + phospholipid
-
16278
Homo sapiens
?
-
-
-
?
2.3.1.148
lyso-Phosphatidylethanolamine + phospholipid
-
16278
Homo sapiens
Phosphatidylethanolamine + lyso-phospholipid
-
-
-
?
2.3.1.148
lyso-Phosphatidylinositol + phospholipid
-
16278
Homo sapiens
Phosphatidylinositol + lyso-phospholipid
-
-
-
?
2.3.1.148
lyso-Phosphatidylserine + phospholipid
-
16278
Homo sapiens
Phosphatidylserine + lyso-phospholipid
-
-
-
?
2.3.1.148
additional information
arachidonate and linoleate at the sn-2 position of phosphatidylcholine can be transferred to lyso-phosphatidylethanolamine. The transfer of 16:0, 18:0, and 18:1 acyl moieties at the sn-2-position of phosphatidylcholine is negligible
16278
Rattus norvegicus
?
-
-
-
?
2.3.1.148
additional information
more efficient transfer of arachidonate from phosphatidylcholine to the ethanolamine-containing phospholipids than from phosphatidylinositol
16278
Homo sapiens
?
-
-
-
?
2.3.1.148
Phosphatidylcholine + lyso-phosphatidylethanolamine
-
16278
Mus musculus
lyso-Phosphatidylcholine + phosphatidylethanolamine
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.148
4.5
-
bimodal pH-optimum, at pH 4.5 and at pH 7.5. The activity is 4-5times higher at pH 4.5 than at pH 7.5
Canis lupus familiaris
2.3.1.148
7.5
-
-
Rattus norvegicus
2.3.1.148
7.5
-
bimodal pH-optimum, at pH 7.5 and at pH 4.5
Canis lupus familiaris