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Literature summary extracted from

  • Zerez, C.R.; Wong, M.D.; Tanaka, K.R.
    Partial purification and properties of nicotinamide adenine dinucleotide synthetase from human erythrocytes: evidence that enzyme activity is a sensitive indicator of lead exposure (1990), Blood, 75, 1576-1582.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.5.1 medicine erythrocyte NAD synthetase is activated by lead, and the activity is a sensitive indicator of lead exposure in human Homo sapiens

General Stability

EC Number General Stability Organism
6.3.5.1 EDTA and KCl increase stability substantially Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.1 Pb2+
-
 Homo sapiens
6.3.5.1 Zn2+
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.1 0.108
-
 deamido-NAD+
-
 Homo sapiens
6.3.5.1 0.154
-
 ATP
-
 Homo sapiens
6.3.5.1 2.17
-
 Gln
-
 Homo sapiens
6.3.5.1 64.2
-
 NH4+
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.1 K+ required Homo sapiens
6.3.5.1 K+ Km: 8.32 mM Homo sapiens
6.3.5.1 Mg2+ required Homo sapiens
6.3.5.1 Mg2+ Km: 1.36 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.1 483000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.1 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.1 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.1 0.017
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.3.5.1 4°C, 24 h, complete loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.1 ATP + deamido-NAD+ + L-Gln
-
 Homo sapiens AMP + diphosphate + NAD+ + L-Glu
-
 ?
6.3.5.1 ATP + deamido-NAD+ + NH4+
-
 Homo sapiens AMP + diphosphate + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.1 6.8 7.4
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.1 6 8 6.0: about 75% of maximal activity, 8.0: about 55% of maximal activity Homo sapiens