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Literature summary extracted from

  • Wagner, R.; Wagner, K.G.
    The pyridine-nucleotide cycle in tobacco. Enzyme activities for the de-novo synthesis of NAD (1985), Planta, 165, 532-537.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.18 phosphate buffer, 0.08 M, pH 7.5, stabilizes Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.1 Mg2+ in excess of ATP Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.18 1.3
-
 nicotinate ribonucleotide 30°C, pH 7.5 Nicotiana tabacum
2.7.7.18 4
-
 ATP 30°C, pH 7.5 Nicotiana tabacum
6.3.5.1 0.083
-
 deamido-NAD+
-
 Nicotiana tabacum
6.3.5.1 0.18
-
 ATP
-
 Nicotiana tabacum
6.3.5.1 2
-
 Gln
-
 Nicotiana tabacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.18 Mg2+ requirement Nicotiana tabacum
6.3.5.1 Mg2+ required Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.18 ATP + nicotinate ribonucleotide Nicotiana tabacum
-
 diphosphate + deamido-NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.18 Nicotiana tabacum
-
 tobacco, cv. Samsun or cv. Xanthi
-
6.3.5.1 Nicotiana tabacum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.18 callus cultured from seedlings' roots Nicotiana tabacum
-
2.7.7.18 root cv. Samsun Nicotiana tabacum
-
6.3.5.1 callus
-
 Nicotiana tabacum
-
6.3.5.1 root
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.18 0.00133
-
 Xanthi, callus culture Nicotiana tabacum
2.7.7.18 0.00269
-
 Samsun, callus culture Nicotiana tabacum
2.7.7.18 0.00358
-
 Samsun, root Nicotiana tabacum

Storage Stability

EC Number Storage Stability Organism
2.7.7.18 frozen 24 h Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.18 ATP + nicotinate ribonucleotide
-
 Nicotiana tabacum diphosphate + deamido-NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Nicotiana tabacum diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
6.3.5.1 ATP + deamido-NAD+ + Asn
-
 Nicotiana tabacum AMP + diphosphate + NAD+ + Asp
-
 ?
6.3.5.1 ATP + deamido-NAD+ + L-Gln
-
 Nicotiana tabacum AMP + diphosphate + NAD+ + L-Glu
-
 ?
6.3.5.1 additional information no activity with NH4+ as nitrogen donor at 3 mM NH4+ Nicotiana tabacum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.18 30
-
 assay at Nicotiana tabacum