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Literature summary extracted from

  • Waheed, A.; Castric, P.A.
    Purification and properties of the urea amidolyase from Candida utilis (1977), J. Biol. Chem., 252, 1628-1632.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.6 10 mM urea plus 25% glycerol protect greatly from thermal inactivation Cyberlindnera jadinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.6 5,5'-dithiobis(2-nitrobenzoate) slight effect Cyberlindnera jadinii
6.3.4.6 NEM slight effect Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.6 70000
-
 2 * 70000 + 2 * 170000, SDS-PAGE, enzyme complex of urea carboxylase and allophanate hydrolase Cyberlindnera jadinii
6.3.4.6 170000
-
 2 * 70000 + 2 * 170000, SDS-PAGE, enzyme complex of urea carboxylase and allophanate hydrolase Cyberlindnera jadinii
6.3.4.6 500000 520000 gel filtration, enzyme complex of urea carboxylase and allophanate hydrolase Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.6 Cyberlindnera jadinii
-
 enzyme complex of urea carboxylase, EC 6.3.4.6, and allophanate hydrolase, EC 3.4.1.13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.6 enzyme complex of urea carboxylase and allophanate hydrolase Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.6 12.5
-
-
 Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.6 ATP + urea + HCO3-
-
 Cyberlindnera jadinii ADP + phosphate + allophanate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.6 tetramer 2 * 70000 + 2 * 170000, SDS-PAGE, enzyme complex of urea carboxylase and allophanate hydrolase Cyberlindnera jadinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.6 30
-
 in absence of stabilizers, half-life: 1 h Cyberlindnera jadinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.4.6 8
-
 marked loss of activity below Cyberlindnera jadinii