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Literature summary extracted from
- Subunit structure and tRNA-binding properties of Bombyx mori glycyl-tRNA synthetase (1985), J. Biochem., 98, 177-186.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 6.1.1.14 | DTT or 25% glycerol stabilizes | Bombyx mori |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.14 | 0.001 | - |
tRNAGly | tRNA2Gly, , aminoacylation | Bombyx mori |  |
| 6.1.1.14 | 0.0026 | - |
tRNA1Gly | aminoacylation | Bombyx mori | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.14 | 90000 | - |
2 * 90000, SDS-PAGE | Bombyx mori |
| 6.1.1.14 | 230000 | - |
gel filtration | Bombyx mori |
| 6.1.1.14 | 270000 | - |
polyacrylamide 4/30% gradient PAGE | Bombyx mori |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.14 | Bombyx mori | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.14 | - |
Bombyx mori |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.1.1.14 | silk gland | posterior | Bombyx mori | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.14 | 1.65 | - |
- |
Bombyx mori |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.14 | ATP + glycine + tRNAGly | - |
Bombyx mori | AMP + diphosphate + glycyl-tRNAGly | - |
? | |
| 6.1.1.14 | additional information | enzyme has two active sites for aminoacylation. tRNA2Gly binds with the enzyme in a molar ratio of 1:1. tRNA2Gly forms a 2:1 complex with the enzyme and is aminoacylated 2fold faster | Bombyx mori | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.1.1.14 | ? | x * 87000-90000, SDS-PAGE, gel filtration in 6 M guanidine-HCl | Bombyx mori |
| 6.1.1.14 | dimer | 2 * 90000, SDS-PAGE | Bombyx mori |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.1.1.14 | 8.1 | - |
tRNA2Gly | aminoacylation | Bombyx mori | |
| 6.1.1.14 | 15.9 | - |
tRNA1Gly | aminoacylation | Bombyx mori | |
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