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Literature summary extracted from

  • Wang, W.; Poland, B.W.; Honzatko, R.B.; Fromm, H.J.
    Identification of arginine residues in the putative L-aspartate binding site of Escherichia coli adenylosuccinate synthetase (1995), J. Biol. Chem., 270, 13160-13168.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.4 R303L mutant enzymes R303L, R304L, and R305L exhibit a 50-200fold increase in their Km values for Asp relative to the wild-type enzyme. The Km values for GTP and IMP are comparable Escherichia coli
6.3.4.4 R304L mutant enzymes R303L, R304L, and R305L exhibit a 50-200fold increase in their Km values for Asp relative to the wild-type enzyme. The Km values for GTP and IMP are comparable Escherichia coli
6.3.4.4 R305L mutant enzymes R303L, R304L, and R305L exhibit a 50-200fold increase in their Km values for Asp relative to the wild-type enzyme. The Km values for GTP and IMP are comparable Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.01
-
 GTP Asp, mutant R305L Escherichia coli
6.3.4.4 0.0201
-
 GTP mutant R303L Escherichia coli
6.3.4.4 0.026
-
 GTP wild-type Escherichia coli
6.3.4.4 0.0265
-
 GTP mutant R304L Escherichia coli
6.3.4.4 0.0278
-
 IMP wild-type Escherichia coli
6.3.4.4 0.0296
-
 IMP mutant R304L Escherichia coli
6.3.4.4 0.0305
-
 IMP mutant R305L Escherichia coli
6.3.4.4 0.0306
-
 GTP mutant R305L Escherichia coli
6.3.4.4 0.0352
-
 IMP mutant R303L Escherichia coli
6.3.4.4 0.057
-
 Asp mutant R303L Escherichia coli
6.3.4.4 0.0657
-
 Asp mutant R303L Escherichia coli
6.3.4.4 0.23
-
 Asp Asp, wild-type Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Escherichia coli
-
 wild-type and mutant enzymes R303L, R304L, and R305L
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4 mutant enzymes R303L, R304L, and R305L Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-Asp
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?