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Literature summary extracted from

  • Dignam, S.S.; Dignam, J.D.
    Glycyl- and alanyl-tRNA synthetases from Bombyx mori. Purification and properties (1984), J. Biol. Chem., 259, 4043-4048.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.7 spermine stimulates only at low Mg2+ concentrations Bombyx mori

General Stability

EC Number General Stability Organism
6.1.1.7 sensitive to repeated freezing and thawing Bombyx mori
6.1.1.14 sensitive to repeated freezing and thawing Bombyx mori
6.1.1.14 unstable after dilution to less than 0.02 mg/ml in absence of nonspecific carrier proteins Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.14 spermine
-
 Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 0.00063
-
 liver tRNA Bombyx mori tRNA Bombyx mori
6.1.1.7 0.34
-
 alanine
-
 Bombyx mori
6.1.1.7 1.7
-
 ATP
-
 Bombyx mori
6.1.1.14 0.0025
-
 tRNAGly substrate from liver Bombyx mori
6.1.1.14 0.0033
-
 tRNAGly substrate from Bombyx mori Bombyx mori
6.1.1.14 0.18
-
 Gly
-
 Bombyx mori
6.1.1.14 0.37
-
 ATP
-
 Bombyx mori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Mg2+ optimum: 5-10 mM Bombyx mori
6.1.1.14 Mg2+ required Bombyx mori
6.1.1.14 Mg2+ optimal concentration is 3-5 mM Bombyx mori
6.1.1.14 additional information the enzyme is twice as active in Tris-HCl or Hepes-NaOH as it is in potassium phosphate buffer Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 110000
-
 1 * 110000, SDS-PAGE Bombyx mori
6.1.1.7 151000
-
 gel filtration Bombyx mori
6.1.1.14 80000
-
 2 * 80000, SDS-PAGE Bombyx mori
6.1.1.14 160000
-
 gel filtration, sedimentation velocity Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + alanine + tRNAAla Bombyx mori key role in protein biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Bombyx mori
-
-
-
6.1.1.14 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7
-
 Bombyx mori
6.1.1.14
-
 Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.7 silk gland posterior Bombyx mori
-
6.1.1.14 silk gland posterior Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.14 additional information
-
-
 Bombyx mori

Storage Stability

EC Number Storage Stability Organism
6.1.1.7 -80°C, 3 months, retains approximately 90% of activity Bombyx mori
6.1.1.14 -80°C, retains approximately 90% of its activity after 3 months Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + alanine + tRNAAla key role in protein biosynthesis Bombyx mori ?
-
 ?
6.1.1.7 ATP + L-alanine + liver tRNA
-
 Bombyx mori ?
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Bombyx mori AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.14 ATP + glycine + tRNAGly
-
 Bombyx mori AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.7 monomer 1 * 110000, SDS-PAGE Bombyx mori
6.1.1.14 dimer 2 * 80000, SDS-PAGE Bombyx mori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.14 additional information
-
 additional information
-
 Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.5
-
 alanyl-tRNA synthesis Bombyx mori
6.1.1.14 8 8.5
-
 Bombyx mori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.7 6.4 7.5 6.4: about 60% of maximal activity, 7.5: optimum Bombyx mori