EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.4 | R143L | mutant enzyme R143L with no change in catalytic constant or Km for Asp, but significantly impaired nucleotide binding, 60fold increased Km for IMP and 10fold increased Km for GTP | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.4 | Guanosine 5'-O-[S-(4-bromo-2,3-dioxobutyl)thio]phosphate | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.4 | 0.026 | - |
GTP | wild-type | Escherichia coli | |
6.3.4.4 | 0.03 | - |
Asp | IMP, wild-type | Escherichia coli | |
6.3.4.4 | 0.23 | - |
Asp | Asp, wild-type | Escherichia coli | |
6.3.4.4 | 0.269 | - |
GTP | mutant R143L | Escherichia coli | |
6.3.4.4 | 0.34 | - |
allopurinol ribonucleotide | Asp, mutant R143L | Escherichia coli | |
6.3.4.4 | 1.73 | - |
IMP | mutant R143L | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.4 | Escherichia coli | - |
wild-type and mutant R143L | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.4 | GTP + 4-hydroxypyrazolo[3,4-d]pyrimidine ribonucleotide + L-Asp | - |
Escherichia coli | GDP + phosphate + 4-aminopyrazolo[3,4-d]pyrimidine ribonucleotide | - |
? | |
6.3.4.4 | GTP + IMP + L-Asp | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.4 | additional information | - |
additional information | - |
Escherichia coli |