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Literature summary extracted from

  • Jahngen, E.G.E.; Rossomando, E.F.
    Adenylosuccinate synthetase from Dictyostelium discoideum: effects of hadacidin analogs and binding of [14C]hadacidin (1984), Arch. Biochem. Biophys., 229, 145-154.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.4 Formycin B monophosphate
-
 Dictyostelium discoideum
6.3.4.4 guanosine 5'-(beta,gamma-imino)triphosphate
-
 Dictyostelium discoideum
6.3.4.4 Hadacidin 5 mM, 100% inhibition Dictyostelium discoideum
6.3.4.4 N-(Thiocarboxy)-L-aspartic anhydride 5 mM, 27.5% inhibition Dictyostelium discoideum
6.3.4.4 N-Acetyl-N-hydroxyglycine 5 mM, 72.9% inhibition Dictyostelium discoideum
6.3.4.4 N-acetylglycine 5 mM, 51.2% inhibition Dictyostelium discoideum
6.3.4.4 N-benzoylglycine 5 mM, 6% inhibition Dictyostelium discoideum
6.3.4.4 N-formylglycine 5 mM, 61.1% inhibition Dictyostelium discoideum
6.3.4.4 N-hydroxyglycine 5 mM, 49.3% inhibition Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.4 0.023
-
 GTP
-
 Dictyostelium discoideum
6.3.4.4 0.036
-
 IMP
-
 Dictyostelium discoideum
6.3.4.4 0.714
-
 Asp
-
 Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.4 Mg2+ required Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4 partial Dictyostelium discoideum

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.4 GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP fully random terter mechanism Dictyostelium discoideum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.4 1.388
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.4 GTP + IMP + L-Asp
-
 Dictyostelium discoideum GDP + phosphate + adenylosuccinate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.4 37
-
-
 Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.4 7
-
-
 Dictyostelium discoideum