EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.4 | cloned into the temperature-inducible, high-copy-number plasmid vector, pMOB45. Upon temperature induction, cells containing this plasmid produce adenylosuccinate synthetase at approximately 40times the wild-type level | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.4 | 0.038 | - |
GTP | - |
Escherichia coli | |
6.3.4.4 | 0.054 | - |
IMP | - |
Escherichia coli | |
6.3.4.4 | 0.98 | - |
L-Asp | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.4 | 48000 | - |
2 * 48000, SDS-PAGE | Escherichia coli |
6.3.4.4 | 141000 | - |
HPLC gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.4 | GTP + IMP + L-Asp | Escherichia coli | plays an important role in the interconversion of purines | ? | - |
? | |
6.3.4.4 | GTP + IMP + L-Asp | Escherichia coli | first committed step towards the de novo biosynthesis of AMP | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.4 | Escherichia coli | - |
cloned into the temperature-inducible, high-copy-number plasmid vector, pMOB45. Upon temperature induction, cells containing this plasmid produce adenylosuccinate synthetase at approximately 40times the wild-type level | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.4 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.4.4 | 1.731 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.4 | GTP + IMP + L-Asp | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? | |
6.3.4.4 | GTP + IMP + L-Asp | plays an important role in the interconversion of purines | Escherichia coli | ? | - |
? | |
6.3.4.4 | GTP + IMP + L-Asp | first committed step towards the de novo biosynthesis of AMP | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.4 | dimer | 2 * 48000, SDS-PAGE | Escherichia coli |