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Literature summary extracted from

  • Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans (1993), J. Bacteriol., 175, 7430-7440.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.83 genetic and sequence analysis Pseudomonas denitrificans (nom. rej.)

General Stability

EC Number General Stability Organism
1.14.13.83 upon handling or storing CobG preparations, the sulfur content, the A400, and the enzymatic activity drops simultaneously Pseudomonas denitrificans (nom. rej.)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.83 Fe2+ iron-sulfur protein Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.131 27000
-
 x * 27000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.133 31000
-
 x * 31000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.152 31000
-
 x * 31000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.83 precorrin-3A + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 precorrin-3B + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3A + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 precorrin-3B + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3A + NADH + O2 Pseudomonas denitrificans (nom. rej.)
-
 precorrin-3B + NAD+ + H2O
-
 ?
1.14.13.83 precorrin-3A + NADH + O2 Pseudomonas denitrificans (nom. rej.) SC510 (RifT)
-
 precorrin-3B + NAD+ + H2O
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B Pseudomonas denitrificans (nom. rej.) the enzyme is involved in the cobalamin biosynthetic pathway ?
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B Pseudomonas denitrificans (nom. rej.) SC510 (RifT) the enzyme is involved in the cobalamin biosynthetic pathway ?
-
 ?
2.1.1.133 S-Adenosyl-L-methionine + precorrin-4 Pseudomonas denitrificans (nom. rej.) enzyme in the biosynthesis of vitamin B12 ?
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O Pseudomonas denitrificans (nom. rej.)
-
 S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O Pseudomonas denitrificans (nom. rej.) SC510 (RifT)
-
 S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
6.6.1.2 ATP + hydrogenobyrinic acid a,c-diamide + Co2+ Pseudomonas denitrificans (nom. rej.)
-
 ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.83 Pseudomonas denitrificans (nom. rej.)
-
-
-
1.14.13.83 Pseudomonas denitrificans (nom. rej.) SC510 (RifT)
-
-
-
2.1.1.131 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.133 Pseudomonas denitrificans (nom. rej.) P21922
-
-
2.1.1.152 Pseudomonas denitrificans (nom. rej.)
-
-
-
6.6.1.2 Pseudomonas denitrificans (nom. rej.)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.83
-
 Pseudomonas denitrificans (nom. rej.)
2.1.1.133
-
 Pseudomonas denitrificans (nom. rej.)
2.1.1.152
-
 Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.83 0.048
-
-
 Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.83 precorrin-3A + NADH + H+ + O2
-
 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3A + NADH + H+ + O2 pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3A + NADH + H+ + O2
-
 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) precorrin-3B + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3A + NADH + H+ + O2 pathway to coenzyme B12, biosynthesis of the corrin macrocycle, catalyzes a complex oxidative reaction involving C20 hydroxylation and gamma-lactone formation from ring-A acetate to C1 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) precorrin-3B + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3A + NADH + O2
-
 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O
-
 ?
1.14.13.83 precorrin-3A + NADH + O2
-
 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) precorrin-3B + NAD+ + H2O
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B the enzyme is involved in the cobalamin biosynthetic pathway Pseudomonas denitrificans (nom. rej.) ?
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B the enzyme is involved in the cobalamin biosynthetic pathway Pseudomonas denitrificans (nom. rej.) SC510 (RifT) ?
-
 ?
2.1.1.133 S-adenosyl-L-methionine + precorrin-4
-
 Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-5
-
 ?
2.1.1.133 S-Adenosyl-L-methionine + precorrin-4 enzyme in the biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) ?
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O
-
 Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O
-
 Pseudomonas denitrificans (nom. rej.) SC510 (RifT) S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O
-
 Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-6x + acetate
-
 ?
6.6.1.2 ATP + hydrogenobyrinic acid a,c-diamide + Co2+
-
 Pseudomonas denitrificans (nom. rej.) ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.131 dimer x * 27000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.133 dimer x * 31000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.152 ? x * 31000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

EC Number Synonyms Comment Organism
1.14.13.83 CobG
-
 Pseudomonas denitrificans (nom. rej.)
2.1.1.152 CobF
-
 Pseudomonas denitrificans (nom. rej.)
2.1.1.152 precorrin-6X synthase (deacetylating)
-
 Pseudomonas denitrificans (nom. rej.)