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Literature summary extracted from

  • Scott, A.I.; Roessner, C.A.; Stolowich, N.J.; Spencer, J.B.; Min, C.; Ozaki, S.I.
    Biosynthesis of vitamin B12. Discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group (1993), FEBS Lett., 331, 105-108.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.83 expressed in Escherichia coli BL21DE3 Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.83 45000
-
 SDS-PAGE Pseudomonas denitrificans (nom. rej.)
1.14.13.83 46990
-
 predicted from the DNA sequence Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.83 precorrin-3 + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) biosynthesis of vitamin B12, part of the ring contractase system for oxidative ring contraction precorrin-3x + NAD+ + H2O
-
 ir
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B Pseudomonas denitrificans (nom. rej.) the enzyme is involved in the cobalamin biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.83 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.131 Pseudomonas denitrificans (nom. rej.)
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.131 S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4 mechanism for ring contraction Pseudomonas denitrificans (nom. rej.)
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.83 precorrin-3 + NADH + H+ + O2
-
 Pseudomonas denitrificans (nom. rej.) precorrin-3x + NAD+ + H2O
-
 ir
1.14.13.83 precorrin-3 + NADH + H+ + O2 biosynthesis of vitamin B12, part of the ring contractase system for oxidative ring contraction Pseudomonas denitrificans (nom. rej.) precorrin-3x + NAD+ + H2O
-
 ir
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B
-
 Pseudomonas denitrificans (nom. rej.) S-Adenosyl-L-homocysteine + precorrin-4
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B the enzyme is involved in the cobalamin biosynthetic pathway Pseudomonas denitrificans (nom. rej.) ?
-
 ?