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Literature summary extracted from

  • Kounga, K.; Song, S.; Haslam, G.C.; Himes, R.H.
    Site-directed mutagenesis of putative catalytic and nucleotide binding sites in N10-formyltetrahydrofolate synthetase (1996), Biochim. Biophys. Acta, 1296, 112-130.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.3 H125S site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion Clostridium cylindrosporum
6.3.4.3 H131S site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion Clostridium cylindrosporum
6.3.4.3 H268S site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion Clostridium cylindrosporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.3 0.13
-
 ADP tetrahydrofolate, mutant enzyme K71Q Clostridium cylindrosporum
6.3.4.3 0.23
-
 tetrahydrofolate MgATP2-, wild-type enzyme Clostridium cylindrosporum
6.3.4.3 0.26
-
 ATP mutant enzyme K71Q Clostridium cylindrosporum
6.3.4.3 0.4
-
 ATP mutant enzyme H131S Clostridium cylindrosporum
6.3.4.3 0.59
-
 ATP mutant enzyme H125S Clostridium cylindrosporum
6.3.4.3 0.69
-
 tetrahydrofolate wild-type enzyme Clostridium cylindrosporum
6.3.4.3 0.97
-
 tetrahydrofolate mutant enzyme H125S Clostridium cylindrosporum
6.3.4.3 1.12
-
 tetrahydrofolate mutant enzyme H131S Clostridium cylindrosporum
6.3.4.3 6.7
-
 formate wild-type enzyme Clostridium cylindrosporum
6.3.4.3 8.2
-
 formate mutant enzyme H125S Clostridium cylindrosporum
6.3.4.3 12.1
-
 formate mutant enzyme K71Q Clostridium cylindrosporum
6.3.4.3 20.2
-
 formate mutant enzyme H131S Clostridium cylindrosporum

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Clostridium cylindrosporum
-
 wild-type and site-directed mutant enzymes H125S, H131S, and H268S
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Clostridium cylindrosporum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.3 additional information
-
 additional information
-
 Clostridium cylindrosporum