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Literature summary extracted from

  • Sato, F.; Takeshita, N.; Fitchen, J.H.; Fujiwara, H.; Yamada, Y.
    S-Adenosyl-L-methionine:scoulerine-9-O-methyltransferase from cultured Coptis japonica cells (1993), Phytochemistry, 32, 659-664.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.117 Berberine 5 mM, 70% inhibition Coptis japonica
2.1.1.117 Ca2+ 1 mM, 64% inhibition Coptis japonica
2.1.1.117 Cu2+ 1 mM, 72% inhibition Coptis japonica
2.1.1.117 Mn2+ 1 mM, 59% inhibition Coptis japonica
2.1.1.117 palmatine 5 mM, 70% inhibition Coptis japonica
2.1.1.117 PCMB 0.5 mM, 51% inhibition Coptis japonica
2.1.1.117 S-adenosylhomocysteine
-
 Coptis japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.117 0.1
-
 (S)-scoulerine
-
 Coptis japonica
2.1.1.117 0.17
-
 S-adenosyl-L-methionine
-
 Coptis japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.117 41000
-
 x * 41000, SDS-PAGE Coptis japonica
2.1.1.117 120000 140000 gel filtration Coptis japonica

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.117 Coptis japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.117
-
 Coptis japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.117 S-Adenosyl-L-methionine + (S)-scoulerine
-
 Coptis japonica S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
 ?
2.1.1.117 S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline 8% of the activity relative to (S)-scoulerine Coptis japonica ?
-
 ?
2.1.1.117 S-Adenosyl-L-methionine + norlaudanosoline 2% of the activity relative to (S)-scoulerine Coptis japonica ?
-
 ?
2.1.1.117 S-Adenosyl-L-methionine + norreticuline 9% of the activity relative to (S)-scoulerine Coptis japonica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.117 ? x * 41000, SDS-PAGE Coptis japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.117 9 9.5
-
 Coptis japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.117 7.5 10 50% of maximal activity at pH 7.5 and at pH 10 Coptis japonica