BRENDA - Enzyme Database

S-Adenosyl-L-methionine:scoulerine-9-O-methyltransferase from cultured Coptis japonica cells

Sato, F.; Takeshita, N.; Fitchen, J.H.; Fujiwara, H.; Yamada, Y.; Phytochemistry 32, 659-664 (1993)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.117
Berberine
5 mM, 70% inhibition
Coptis japonica
2.1.1.117
Ca2+
1 mM, 64% inhibition
Coptis japonica
2.1.1.117
Cu2+
1 mM, 72% inhibition
Coptis japonica
2.1.1.117
Mn2+
1 mM, 59% inhibition
Coptis japonica
2.1.1.117
palmatine
5 mM, 70% inhibition
Coptis japonica
2.1.1.117
PCMB
0.5 mM, 51% inhibition
Coptis japonica
2.1.1.117
S-adenosylhomocysteine
-
Coptis japonica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.117
0.1
-
scoulerine
-
Coptis japonica
2.1.1.117
0.17
-
S-adenosyl-L-methionine
-
Coptis japonica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.117
41000
-
x * 41000, SDS-PAGE
Coptis japonica
2.1.1.117
120000
140000
gel filtration
Coptis japonica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.117
Coptis japonica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.117
-
Coptis japonica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.117
S-Adenosyl-L-methionine + (S)-scoulerine
-
15193
Coptis japonica
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
2.1.1.117
S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline
8% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
2.1.1.117
S-Adenosyl-L-methionine + norlaudanosoline
2% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
2.1.1.117
S-Adenosyl-L-methionine + norreticuline
9% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.117
?
x * 41000, SDS-PAGE
Coptis japonica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.117
9
9.5
-
Coptis japonica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.117
7.5
10
50% of maximal activity at pH 7.5 and at pH 10
Coptis japonica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.117
Berberine
5 mM, 70% inhibition
Coptis japonica
2.1.1.117
Ca2+
1 mM, 64% inhibition
Coptis japonica
2.1.1.117
Cu2+
1 mM, 72% inhibition
Coptis japonica
2.1.1.117
Mn2+
1 mM, 59% inhibition
Coptis japonica
2.1.1.117
palmatine
5 mM, 70% inhibition
Coptis japonica
2.1.1.117
PCMB
0.5 mM, 51% inhibition
Coptis japonica
2.1.1.117
S-adenosylhomocysteine
-
Coptis japonica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.117
0.1
-
scoulerine
-
Coptis japonica
2.1.1.117
0.17
-
S-adenosyl-L-methionine
-
Coptis japonica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.117
41000
-
x * 41000, SDS-PAGE
Coptis japonica
2.1.1.117
120000
140000
gel filtration
Coptis japonica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.117
-
Coptis japonica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.117
S-Adenosyl-L-methionine + (S)-scoulerine
-
15193
Coptis japonica
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
2.1.1.117
S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline
8% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
2.1.1.117
S-Adenosyl-L-methionine + norlaudanosoline
2% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
2.1.1.117
S-Adenosyl-L-methionine + norreticuline
9% of the activity relative to (S)-scoulerine
15193
Coptis japonica
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.117
?
x * 41000, SDS-PAGE
Coptis japonica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.117
9
9.5
-
Coptis japonica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.117
7.5
10
50% of maximal activity at pH 7.5 and at pH 10
Coptis japonica