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Literature summary extracted from

  • Frenzel, T.; Zenk, M.H.
    S-Adenosyl-L-methionine: 3'-hydroxy-N-methyl-(S)-coclaurine-4'-O-methyl transferase, a regio- and stereoselective enzyme of the (S)-reticuline pathway (1990), Phytochemistry, 29, 3505-3511.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.116 (R)-3'-Hydroxy-coclaurine
-
 Berberis koetineana
2.1.1.116 (S)-3'-hydroxy-coclaurine
-
 Berberis koetineana
2.1.1.116 (S)-norreticuline
-
 Berberis koetineana
2.1.1.116 (S)-reticuline
-
 Berberis koetineana
2.1.1.116 3'-Hydroxy-N-methyl-(R)-coclaurine
-
 Berberis koetineana
2.1.1.116 Berberine
-
 Berberis koetineana
2.1.1.116 Jatrorrhizine
-
 Berberis koetineana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.116 0.0045
-
 3'-hydroxy-N-methyl-(S)-coclaurine
-
 Berberis koetineana
2.1.1.116 0.03
-
 S-adenosyl-L-methionine
-
 Berberis koetineana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.116 40000
-
 1 * 40000, SDS-PAGE Berberis koetineana
2.1.1.116 51000
-
 gel filtration Berberis koetineana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine Berberis koetineana the enzyme catalyzes the last step in (S)-reticuline biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.116 Berberis koetineana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.116
-
 Berberis koetineana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.116 cell culture
-
 Berberis koetineana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.116 additional information
-
-
 Berberis koetineana

Storage Stability

EC Number Storage Stability Organism
2.1.1.116 -20°C, 20% glycerol, stable for more than 1 year Berberis koetineana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.116 S-Adenosyl-L-methionine + (R,S)-7-O-methyllaudanosoline 93% of the activity relative to 3'-hydroxy-N-methyl-(S)-coclaurine Berberis koetineana S-Adenosyl-L-homocysteine + norprotosinomenine
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + (R,S)-laudanosoline 84% of the activity relative to 3'-hydroxy-N-methyl-(S)-coclaurine Berberis koetineana S-Adenosyl-L-homocysteine + 4'-O-methyllaudanosoline
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + (S)-3'-hydroxy-coclaurine 10% of the activity relative to 3'-hydroxy-N-methyl-(S)-coclaurine Berberis koetineana S-Adenosyl-L-homocysteine + (S)-norreticuline
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
 Berberis koetineana S-Adenosyl-L-homocysteine + (S)-reticuline
-
 ?
2.1.1.116 S-Adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine the enzyme catalyzes the last step in (S)-reticuline biosynthesis Berberis koetineana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.116 monomer 1 * 40000, SDS-PAGE Berberis koetineana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.116 35 40
-
 Berberis koetineana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.116 6
-
 half-life: 15 days Berberis koetineana
2.1.1.116 20
-
 half-life: 3 days Berberis koetineana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.116 8.3 8.5
-
 Berberis koetineana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.116 7 9 about 50% of maximal activity at pH 7 and at pH 9 Berberis koetineana