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Literature summary extracted from

  • Cossins, E.A.; Kirk, C.D.; Imeson, H.C.; Zheng, L.l.
    Enzymes for synthesis of 10-formyltetrahydrofolate in plants. Characterization of a monofunctional 10-formyltetrahydrofolate synthetase and copurification of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methenyltetrahydrofolate cyclohydrolase activities (1993), Adv. Exp. Med. Biol., 338, 707-710.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.0077
-
 tetrahydropteroylglutamic acid
-
 Pisum sativum
1.5.1.5 0.011
-
 NADP+
-
 Pisum sativum
6.3.4.3 0.003
-
 5,6,7,8-tetrahydropteroylpentaglutamate
-
 Pisum sativum
6.3.4.3 0.012
-
 ATP reaction with tetrahydropteroyl-Glu5 Pisum sativum
6.3.4.3 0.04
-
 tetrahydrofolate
-
 Pisum sativum
6.3.4.3 0.094
-
 ATP reaction with tetrahydrofolate Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.5 cytosol
-
 Pisum sativum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.5 57000
-
 1 * 57000, bifunctional protein: methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, SDS-PAGE Pisum sativum
1.5.1.5 60000
-
 methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase protein, gel filtration Pisum sativum
3.5.4.9 57000
-
 SDS-PAGE Pisum sativum
3.5.4.9 60000
-
 gel filtration Pisum sativum
6.3.4.3 56000
-
 2 * 56000, SDS-PAGE Pisum sativum
6.3.4.3 110000
-
 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Pisum sativum
-
 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Pisum sativum
-
 L. cv. Homesteader
-
3.5.4.9 Pisum sativum
-
 L. cv. Homesteader
-
6.3.4.3 Pisum sativum
-
 monofunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 co-purification of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methenyltetrahydrofolate cyclohydrolase Pisum sativum
3.5.4.9
-
 Pisum sativum
6.3.4.3
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.5 cotyledon
-
 Pisum sativum
-
3.5.4.9 cotyledon
-
 Pisum sativum
-
6.3.4.3 cotyledon
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.5 32.5
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 tetrahydropteroylglutamic acid + NADP+
-
 Pisum sativum ?
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Pisum sativum 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + 5,6,7,8-tetrahydropteroylpentaglutamate
-
 Pisum sativum ADP + phosphate + 10-formyltetrahydrofolylpentaglutamate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Pisum sativum ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydropteroyl-(Glu)n n: 1-5 Pisum sativum ADP + phosphate + 10-formyltetrahydropteroyl-(Glu)n
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.5 monomer 1 * 57000, bifunctional protein: methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, SDS-PAGE Pisum sativum
6.3.4.3 dimer 2 * 56000, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.5 6.5
-
-
 Pisum sativum
3.5.4.9 6.5
-
-
 Pisum sativum
6.3.4.3 7.5
-
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Pisum sativum