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Literature summary extracted from

  • Conradt, H.; Hohmann-Berger, M.; Hohmann, H.P.; Blaschkowski, H.P.; Knappe, J.
    Pyruvate formate-lyase (inactive form) and pyruvate formate-lyase activating enzyme of Escherichia coli: isolation and structural properties (1984), Arch. Biochem. Biophys., 228, 133-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.97.1.4 additional information contains a covalently bound chromophoric factor which has an optical absorptiion peak at 388 nm Escherichia coli
2.3.1.54 S-adenosylmethionine
-
Escherichia coli
2.3.1.54 S-adenosylmethionine
-
Streptococcus mutans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.54 Escherichia coli SP 264 contains plasmid p29with pfl gene, identification of pfl gene product as a 80kDa polypeptide Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 iodoacetate
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.4 29500
-
1 * 29500, SDS-PAGE Escherichia coli
1.97.1.4 34000
-
gel filtration Escherichia coli
2.3.1.54 80000
-
identification of pfl gene product Escherichia coli
2.3.1.54 85000
-
2 * 85000, homodimer, SDS-PAGE Escherichia coli
2.3.1.54 85500
-
2 * 85500, homodimer, SDS-PAGE Escherichia coli
2.3.1.54 151000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Streptococcus mutans
-
acetyl-CoA + formate
-
?
2.3.1.54 pyruvate + CoA Escherichia coli key enzyme of the glucose fermentation route in anaerobically growing cells acetyl-CoA + formate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.4 Escherichia coli
-
-
-
2.3.1.54 Escherichia coli
-
K12
-
2.3.1.54 Escherichia coli
-
ATCC 10798
-
2.3.1.54 Streptococcus mutans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.97.1.4
-
Escherichia coli
2.3.1.54
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.97.1.4 0.017
-
-
Escherichia coli
2.3.1.54 160
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.54 -20°C, 10 mM MOPS-KOH, pH 7.0, 50 mM KCl, thiols, EDTA in 50% ethyleneglycol, proved stable for months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.4 S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine formate acetyltransferase-glycine is the inactive form of the enzyme Escherichia coli 5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl-radical formate acetyltransferase-glycine-2-yl-radical is the active form of the enzyme ?
2.3.1.54 pyruvate + CoA
-
Escherichia coli acetyl-CoA + formate
-
?
2.3.1.54 pyruvate + CoA
-
Streptococcus mutans acetyl-CoA + formate
-
?
2.3.1.54 pyruvate + CoA key enzyme of the glucose fermentation route in anaerobically growing cells Escherichia coli acetyl-CoA + formate
-
?

Subunits

EC Number Subunits Comment Organism
1.97.1.4 monomer 1 * 29500, SDS-PAGE Escherichia coli
2.3.1.54 dimer
-
Streptococcus mutans
2.3.1.54 dimer 2 * 85000, homodimer, SDS-PAGE Escherichia coli
2.3.1.54 dimer 2 * 85500, homodimer, SDS-PAGE Escherichia coli