EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.97.1.4 | additional information | contains a covalently bound chromophoric factor which has an optical absorptiion peak at 388 nm | Escherichia coli | |
2.3.1.54 | S-adenosylmethionine | - |
Escherichia coli | |
2.3.1.54 | S-adenosylmethionine | - |
Streptococcus mutans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.54 | Escherichia coli SP 264 contains plasmid p29with pfl gene, identification of pfl gene product as a 80kDa polypeptide | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.54 | iodoacetate | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.97.1.4 | 29500 | - |
1 * 29500, SDS-PAGE | Escherichia coli |
1.97.1.4 | 34000 | - |
gel filtration | Escherichia coli |
2.3.1.54 | 80000 | - |
identification of pfl gene product | Escherichia coli |
2.3.1.54 | 85000 | - |
2 * 85000, homodimer, SDS-PAGE | Escherichia coli |
2.3.1.54 | 85500 | - |
2 * 85500, homodimer, SDS-PAGE | Escherichia coli |
2.3.1.54 | 151000 | - |
sedimentation equilibrium | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.54 | pyruvate + CoA | Streptococcus mutans | - |
acetyl-CoA + formate | - |
? | |
2.3.1.54 | pyruvate + CoA | Escherichia coli | key enzyme of the glucose fermentation route in anaerobically growing cells | acetyl-CoA + formate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.97.1.4 | Escherichia coli | - |
- |
- |
2.3.1.54 | Escherichia coli | - |
K12 | - |
2.3.1.54 | Escherichia coli | - |
ATCC 10798 | - |
2.3.1.54 | Streptococcus mutans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.97.1.4 | - |
Escherichia coli |
2.3.1.54 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.97.1.4 | 0.017 | - |
- |
Escherichia coli |
2.3.1.54 | 160 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.54 | -20°C, 10 mM MOPS-KOH, pH 7.0, 50 mM KCl, thiols, EDTA in 50% ethyleneglycol, proved stable for months | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.97.1.4 | S-adenosyl-L-methionine + dihydroflavodoxin + formate acetyltransferase-glycine | formate acetyltransferase-glycine is the inactive form of the enzyme | Escherichia coli | 5'-deoxyadenosine + methionine + flavodoxin + formate acetyltransferase-glycine-2-yl-radical | formate acetyltransferase-glycine-2-yl-radical is the active form of the enzyme | ? | |
2.3.1.54 | pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
2.3.1.54 | pyruvate + CoA | - |
Streptococcus mutans | acetyl-CoA + formate | - |
? | |
2.3.1.54 | pyruvate + CoA | key enzyme of the glucose fermentation route in anaerobically growing cells | Escherichia coli | acetyl-CoA + formate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.97.1.4 | monomer | 1 * 29500, SDS-PAGE | Escherichia coli |
2.3.1.54 | dimer | - |
Streptococcus mutans |
2.3.1.54 | dimer | 2 * 85000, homodimer, SDS-PAGE | Escherichia coli |
2.3.1.54 | dimer | 2 * 85500, homodimer, SDS-PAGE | Escherichia coli |