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Literature summary extracted from

  • Ostrem, D.L.; Berg, P.
    Glycyl transfer ribonucleic acid synthetase from Escherichia coli. Purification, properties, and substrate binding (1974), Biochemistry, 13, 1338-1348.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.14 aminoethyl-AMP protects from heat inactivation Escherichia coli
6.1.1.14 glycerol required for stabilization Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.14 Aminoethyl-AMP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.14 0.0002
-
 tRNAGly glycyl-tRNA formation Escherichia coli
6.1.1.14 0.014
-
 ATP ATP, , ATP-diphosphate exchange, without tRNA Escherichia coli
6.1.1.14 0.03
-
 Gly ATP-diphosphate exchange, without tRNA Escherichia coli
6.1.1.14 0.04
-
 ATP ATP-diphosphate exchange in presence of tRNA Escherichia coli
6.1.1.14 0.042
-
 ATP glycyl-tRNA formation Escherichia coli
6.1.1.14 0.14
-
 Gly ATP-diphosphate exchange in presence of tRNA Escherichia coli
6.1.1.14 0.16
-
 Gly glycyl-tRNA formation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.14 33000
-
 2 * 33000 (alpha) + 2 * 80000 (beta), SDS-PAGE Escherichia coli
6.1.1.14 80000
-
 2 * 33000 (alpha) + 2 * 80000 (beta), SDS-PAGE Escherichia coli
6.1.1.14 227000
-
 sedimentation equilibrium ultracentrifugation Escherichia coli
6.1.1.14 240000
-
 PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.14
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.14 8.7
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.1.1.14 -15°C, enzyme concentration 0.5 mg/ml, 40% glycerol, 30% loss of activity after 8 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly binds two molecules of glycyl-AMP per tetramer Escherichia coli AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.14 tetramer 2 * 33000 (alpha) + 2 * 80000 (beta), SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.14 50
-
 80% loss of activity after 1 min in absence of substrates, 60% loss of activity after 10 min in presence of aminoethyl-AMP and Mg2+ Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.14 additional information
-
 additional information
-
 Escherichia coli