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Literature summary extracted from

  • Landman, A.D.; Dakshinamurti, K.
    Enzymatic diagnosis of holocarboxylase synthetase deficiency using apo-carboxyl carrier protein as a substrate (1996), Clin. Chim. Acta, 251, 41-52.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.15 analysis biotinylation of apo-carboxyl carrier protein, a subunit of acetyl-CoA carboxylase from E. coli is a sensitive and convenient assay method for biotin-[acetyl-CoA-carboxylase] ligase Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.15 0.26
-
biotin reaction with ATP + apo-carboxyl carrier protein, a subunit of acetyl-CoA carboxylase from E. coli Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.15 fibroblast cultured Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] incorporation of biotin into apo-carboxyl carrier protein, a subunit of acetyl-CoA carboxylase from E. coli Homo sapiens AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
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