BRENDA - Enzyme Database show

Acetyl coenzyme A carboxylase system of Escherichia coli. Purification and properties of the biotin carboxylase, carboxyltransferase, and carboxyl carrier protein components

Guchhait, R.B.; Polakis, S.E.; Dimroth, P.; Stoll, E.; Moss, J.; Lane, M.D.; J. Biol. Chem. 249, 6633-6645 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.4.14
ethanol
maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v
Escherichia coli
Crystallization (Commentary)
EC Number
Crystallization
Organism
6.3.4.14
-
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.14
Co2+
above 2 mM
Escherichia coli
6.3.4.14
ethanol
maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v
Escherichia coli
6.3.4.14
Mn2+
above 2 mM
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.14
Co2+
divalent metal ion required, Mg2+, Mn2+ or Co2+
Escherichia coli
6.3.4.14
Mg2+
divalent metal ion required, Mg2+, Mn2+ or Co2+
Escherichia coli
6.3.4.14
Mn2+
divalent metal ion required, Mg2+, Mn2+ or Co2+
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.14
50000
-
2 * 50000, SDS-PAGE
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.4.14
Escherichia coli
-
B
-
6.3.4.14
Escherichia coli B / ATCC 11303
-
B
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.4.14
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.14
additional information
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.14
ATP + biotin-carboxyl-carrier protein + HCO3-
-
1426
Escherichia coli
ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
1426
Escherichia coli
-
6.3.4.14
ATP + biotin-carboxyl-carrier protein + HCO3-
-
1426
Escherichia coli B / ATCC 11303
ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
1426
Escherichia coli B / ATCC 11303
-
6.3.4.14
ATP + D-(+)-biotin + HCO3-
L-biotin is completely inactive
1426
Escherichia coli
ADP + phosphate + carboxybiotin
-
-
-
-
6.3.4.14
ATP + D-(+)-biotin + HCO3-
L-biotin is completely inactive
1426
Escherichia coli B / ATCC 11303
ADP + phosphate + carboxybiotin
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.3.4.14
dimer
2 * 50000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.14
6.5
-
Mn2+-activated reaction
Escherichia coli
6.3.4.14
8
-
Mg2+-activated reaction
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.4.14
ethanol
maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
6.3.4.14
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.14
Co2+
above 2 mM
Escherichia coli
6.3.4.14
ethanol
maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v
Escherichia coli
6.3.4.14
Mn2+
above 2 mM
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.14
Co2+
divalent metal ion required, Mg2+, Mn2+ or Co2+
Escherichia coli
6.3.4.14
Mg2+
divalent metal ion required, Mg2+, Mn2+ or Co2+
Escherichia coli
6.3.4.14
Mn2+
divalent metal ion required, Mg2+, Mn2+ or Co2+
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.14
50000
-
2 * 50000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.14
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.14
additional information
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.14
ATP + biotin-carboxyl-carrier protein + HCO3-
-
1426
Escherichia coli
ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
1426
Escherichia coli
-
6.3.4.14
ATP + biotin-carboxyl-carrier protein + HCO3-
-
1426
Escherichia coli B / ATCC 11303
ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
1426
Escherichia coli B / ATCC 11303
-
6.3.4.14
ATP + D-(+)-biotin + HCO3-
L-biotin is completely inactive
1426
Escherichia coli
ADP + phosphate + carboxybiotin
-
-
-
-
6.3.4.14
ATP + D-(+)-biotin + HCO3-
L-biotin is completely inactive
1426
Escherichia coli B / ATCC 11303
ADP + phosphate + carboxybiotin
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.4.14
dimer
2 * 50000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.14
6.5
-
Mn2+-activated reaction
Escherichia coli
6.3.4.14
8
-
Mg2+-activated reaction
Escherichia coli