EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.14 | additional information | truncated enzyme forms with deletions of 12, 27, 46, and 55 N-terminal residues reduce the kcat value of the wild-type enzyme by a factor 5-10 in diphosphate exchange and aminoacylation activity, but does not significantly change the Km of the three substrates. Deletions of 108 N-terminal residues or the internal segments 111-164 and 110-309 cause complete loss of activity. Deletions from the C-terminus of 24, 38, 60, 163, and 328 residues result in inactive enzyme forms. Whereas the wild-type enzyme binds both tRNAGly and noncognate tRNAAla, the mutant lacking 55 N-terminal residues shows altered binding of tRNAGly and does not bind tRNAAla | Bombyx mori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.14 | 0.00035 | - |
Gly | wild-type enzyme | Bombyx mori | |
6.1.1.14 | 0.00055 | - |
Gly | N55-truncation mutant | Bombyx mori | |
6.1.1.14 | 0.001 | - |
tRNAGly | N55-truncation mutant enzyme | Bombyx mori | |
6.1.1.14 | 0.0012 | - |
tRNAGly | wild-type enzyme | Bombyx mori | |
6.1.1.14 | 0.013 | - |
ATP | wild-type enzyme | Bombyx mori | |
6.1.1.14 | 0.014 | - |
ATP | N55-truncation mutant enzyme | Bombyx mori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.14 | 76919 | - |
x * 76919, wild-type enzyme, calculation from nucleotide sequence | Bombyx mori |
6.1.1.14 | 155000 | - |
gel filtration, calculation from sedimentation constant | Bombyx mori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.14 | Bombyx mori | - |
wild-type and truncated enzyme forms | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.14 | ATP + glycine + tRNAGly | the wild-type enzyme binds both tRNAGly and noncognate tRNAAla. The mutant lacking 55 N-terminal residues shows altered binding of tRNAGly and does not bind tRNAAla | Bombyx mori | AMP + diphosphate + glycyl-tRNAGly | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.14 | ? | x * 76919, wild-type enzyme, calculation from nucleotide sequence | Bombyx mori |