BRENDA - Enzyme Database show

Purification and properties of bovine liver holocarboxylase synthetase

Chiba, Y.; Suzuki, Y.; Aoki, Y.; Ishida, Y.; Narisawa, K.; Arch. Biochem. Biophys. 313, 8-14 (1994)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.10
0.000013
-
biotin
-
Bos taurus
6.3.4.10
0.02
-
ATP
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.4.10
cytosol
-
Bos taurus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.10
60000
-
gel filtration
Bos taurus
6.3.4.10
64000
-
1 * 64000, SDS-PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.4.10
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.4.10
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.10
0.514
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
biotinylates apopropionyl-CoA carboxylase from patients with holocarboxylase synthetase deficiency
1395
Bos taurus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1395
Bos taurus
-
6.3.4.10
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
117% of the activity relative to ATP
1395
Bos taurus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
46% of the activity relative to ATP
1395
Bos taurus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
47% of the activity relative to ATP
1395
Bos taurus
IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.3.4.10
monomer
1 * 64000, SDS-PAGE
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.10
0.000013
-
biotin
-
Bos taurus
6.3.4.10
0.02
-
ATP
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.4.10
cytosol
-
Bos taurus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.10
60000
-
gel filtration
Bos taurus
6.3.4.10
64000
-
1 * 64000, SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.10
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.10
0.514
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
biotinylates apopropionyl-CoA carboxylase from patients with holocarboxylase synthetase deficiency
1395
Bos taurus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1395
Bos taurus
-
6.3.4.10
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
117% of the activity relative to ATP
1395
Bos taurus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
46% of the activity relative to ATP
1395
Bos taurus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
47% of the activity relative to ATP
1395
Bos taurus
IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.4.10
monomer
1 * 64000, SDS-PAGE
Bos taurus