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Literature summary extracted from

  • Suzuki, Y.; Narisawa, K.
    Purification and properties of bovine and human holocarboxylase synthetase (1997), Methods Enzymol., 279, 386-393.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.10 diagnostics the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency Homo sapiens
6.3.4.10 diagnostics the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.10
-
 Homo sapiens

General Stability

EC Number General Stability Organism
6.3.4.10 after separation of the 64 kDa protein from an inert protein of 34 kDa on a nondenaturing PAGE, HCS is extremely labile and its enzymatic activity is totally lost within a few hours Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.10 0.000013
-
 biotin
-
 Bos taurus
6.3.4.10 0.000013
-
 biotin
-
 Oryctolagus cuniculus
6.3.4.10 0.000013
-
 biotin pH 8, 30°C, cosubstrate apo-PCC from biotin-deficient rat liver Bos taurus
6.3.4.10 20
-
 ATP pH 8, 30°C, cosubstrate apo-PCC from biotin-deficient rat liver Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.10 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.10 64000
-
 gel filtration, HCS seems to be partially degraded, the molecular mass may be 81-85 kDa, the 64 kDa protein purified through gel filtration is clearly separated from an inert protein of 34 kDa on a nondenaturing PAGE at pH 9.5 or 8 Bos taurus
6.3.4.10 80759
-
 x * 80759, calculation from nucleotide sequence Homo sapiens
6.3.4.10 80759
-
 1 * 80759, amino acid sequence calculation Homo sapiens
6.3.4.10 81000 85000
-
 Homo sapiens
6.3.4.10 81000 85000
-
 Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.10 Bos taurus
-
-
-
6.3.4.10 Homo sapiens
-
-
-
6.3.4.10 Oryctolagus cuniculus
-
-
-
6.3.4.10 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.10
-
 Homo sapiens
6.3.4.10
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.10 liver
-
 Rattus norvegicus
-
6.3.4.10 liver
-
 Bos taurus
-
6.3.4.10 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.10 additional information
-
-
 Bos taurus
6.3.4.10 additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Homo sapiens AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Rattus norvegicus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Bos taurus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Oryctolagus cuniculus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC Homo sapiens AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC from biotin-deficient rat liver or human lymphoblast in the presence of d-biotin and ATP, much higher activity with apo-PCC from rat livers than from human lymphoblasts Bos taurus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apocarboxyl carrier protein HCS catalyzes the incorporation of biotin into carboxylases, recombinant apo-CCP from Escherichia coli Bos taurus AMP + diphosphate + holocarboxyl carrier protein
-
 ?
6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Oryctolagus cuniculus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] as active as ATP Bos taurus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 CTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] CTP can totally replace ATP Bos taurus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Oryctolagus cuniculus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 50% of the activity relative to ATP Bos taurus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 GTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] GTP is approximately half as active as ATP Bos taurus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 50% of the activity relative to ATP Bos taurus IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ITP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] ITP is approximately half as active as ATP Bos taurus IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.10 ? x * 80759, calculation from nucleotide sequence Homo sapiens
6.3.4.10 monomer 1 * 80759, amino acid sequence calculation Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.10 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.10 8
-
 assay at Bos taurus