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Literature summary extracted from

  • Lumsden, J.; Coggins, J.R.
    The subunit structure of the arom multienzyme complex of Neurospora crassa. A possible pentafunctional polypeptide chain (1977), Biochem. J., 161, 599-607.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.71 additional information
-
 aggregate containing 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, ED 4.2.3.4 and EC 2.5.1.19 has a MW of 270000 Da determined by glycerol-density-gradient centrifugation Neurospora crassa
4.2.3.4 165000
-
 2 * 165000, SDS-electrophoresis Neurospora crassa
4.2.3.4 290000 330000 gel electrophoresis after cross-linkage with dimethyl suberimidate Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Neurospora crassa
-
 enzyme aggregate contains 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, EC 4.2.3.4 and EC 2.5.1.19
-
4.2.3.4 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.4 arom-multienzyme complex Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.4 additional information
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
2.7.1.71 -20°C, 50% glycerol, aggregate containing 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, 5-dehydroquinate synthase and 3-enoyl-pyruvylshikimate 5-phosphate synthase is stable for at least 1 month Neurospora crassa
2.7.1.71 4°C, 50% glycerol, aggregate containing 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, 5-dehydroquinate synthase and 3-enoyl-pyruvylshikimate 5-phosphate synthase is stable for at least 19 days Neurospora crassa
4.2.3.4 50% glycerol Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Neurospora crassa ADP + shikimate 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.71 More aggregate containing 5 activities: EC 1.1.1.25, EC 2.7.1.71, EC 4.2.1.10, EC 4.2.3.4 and EC 2.5.1.19 is composed of two subunits of 165000 Da Neurospora crassa
4.2.3.4 dimer 2 * 165000, SDS-electrophoresis Neurospora crassa