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Literature summary extracted from
- Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: cloning, expression, and functional characterization of conserved aspartic acid residues (1998), Biochemistry, 37, 10074-10086.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.3 | - |
Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.3.3 | D101E | reduced ratio of turnover-numer:Km-value by about 10000fold compared to the wild-type enzyme | Escherichia coli |
| 4.2.3.3 | D101N | reduced ratio of turnover-numer:Km-value by about 10000fold compared to the wild-type enzyme | Escherichia coli |
| 4.2.3.3 | D71E | reduced ratio of turnover-numer:Km-value by about 1000fold compared to the wild-type enzyme | Escherichia coli |
| 4.2.3.3 | D71N | reduced ratio of turnover-numer:Km-value by about 1000fold compared to the wild-type enzyme | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.3 | 2-phosphoglycerate | - |
Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.3 | 0.2 | - |
dihydroxyacetone phosphate | wild-type enzyme | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.3 | 17000 | - |
4 * 17000, SDS-PAGE | Escherichia coli |
| 4.2.3.3 | 67000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.3 | Escherichia coli | - |
wild-type enzyme and mutant enzymes D71E, D71N, D101E and D101N | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.3 | dihydroxyacetone phosphate | - |
Escherichia coli | methylglyoxal + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.3 | tetramer | 4 * 17000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.3 | 220 | - |
dihydroxyacetone phosphate | wild-type enzyme | Escherichia coli | |
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