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Literature summary extracted from

  • Yuan, P.M.; Gracy, R.W.
    The conversion of dihydroxyacetone phosphate to methylglyoxal and inorganic phosphate by methylglyoxal synthase (1977), Arch. Biochem. Biophys., 183, 1-6.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.3.3 phosphate stabilizes towards both cold-induced inactivation and against heat-induced inactivation Proteus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.3 methylglyoxal linear noncompetitive inhibitor Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.3 Proteus vulgaris
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.3 glycerone phosphate = 2-oxopropanal + phosphate stereospecific deprotonation of the pro-S hydrogen at C-3 of dihydroxyacetone, the true product of the enzymatic reaction is the enol form of methylglyoxal which is ketonized in solution Proteus vulgaris
a

Storage Stability

EC Number Storage Stability Organism
4.2.3.3 4°C, 10 mM imidazole buffer, pH 7.0, 1 mM EDTA, 1 mM KH2PO4, 0.05% v/v 2-mercaptoethanol, 0.1-0.5 mg/ml protein, stable Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.3 dihydroxyacetone phosphate
-
 Proteus vulgaris methylglyoxal + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.3 additional information
-
 phosphate stabilizes towards both cold-induced inactivation and against heat-induced inactivation Proteus vulgaris