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Literature summary extracted from

  • Cooper, R.A.
    Methylglyoxal synthase (1975), Methods Enzymol., 41B, 502-508.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.3.3 removal of phosphate from the purified enzyme leads to rapid loss of activity, which is more pronounced at 0°C than at 22°C. Dihydroxyacetone phosphate and bovine serum albumin overcome the inactivation to some extent Pelomonas saccharophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.3 3-phosphoglycerate
-
 Pelomonas saccharophila
4.2.3.3 diphosphate
-
 Pelomonas saccharophila
4.2.3.3 phosphoenolpyruvate
-
 Pelomonas saccharophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.3 0.09
-
 dihydroxyacetone phosphate
-
 Pelomonas saccharophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.3 67000
-
 gel filtration Pelomonas saccharophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.3 dihydroxyacetone phosphate Pelomonas saccharophila constitutive enzyme, may be involved in by-pass sequence for part of the Embden-Meyerhof glycolytic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.3 Aeromonas caviae
-
-
-
4.2.3.3 Clostridium pasteurianum
-
-
-
4.2.3.3 Clostridium tetanomorphum
-
-
-
4.2.3.3 Obesumbacterium proteus
-
-
-
4.2.3.3 Pelomonas saccharophila
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.3 additional information
-
-
 Pelomonas saccharophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.3 dihydroxyacetone phosphate
-
 Clostridium pasteurianum methylglyoxal + phosphate
-
 ?
4.2.3.3 dihydroxyacetone phosphate
-
 Aeromonas caviae methylglyoxal + phosphate
-
 ?
4.2.3.3 dihydroxyacetone phosphate
-
 Pelomonas saccharophila methylglyoxal + phosphate
-
 ir
4.2.3.3 dihydroxyacetone phosphate
-
 Clostridium tetanomorphum methylglyoxal + phosphate
-
 ?
4.2.3.3 dihydroxyacetone phosphate
-
 Obesumbacterium proteus methylglyoxal + phosphate
-
 ?
4.2.3.3 dihydroxyacetone phosphate constitutive enzyme, may be involved in by-pass sequence for part of the Embden-Meyerhof glycolytic pathway Pelomonas saccharophila ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.3 8.2
-
-
 Pelomonas saccharophila