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Literature summary extracted from

  • Mukhopadhyay, B.; Purwantini, E.; Pihl, T.D.; Reeve, J.N.; Daniels, L.
    Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coli (1995), J. Biol. Chem., 270, 2827-2832.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.98.1 expression in Escherichia coli Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 Methanothermobacter thermautotrophicus
-
-
-
1.5.98.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
 Methanothermobacter thermautotrophicus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.1 45 55 recombinant enzyme Methanothermobacter thermautotrophicus
1.5.98.1 55 65 native enzyme Methanothermobacter thermautotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.98.1 25
-
 stable for more than 70 h, native and recombinant enzyme Methanothermobacter thermautotrophicus
1.5.98.1 40
-
 stable for more than 70 h, native and recombinant enzyme Methanothermobacter thermautotrophicus
1.5.98.1 65
-
 45% loss of activity in 1.5 h, 93% loss of activity in 24 h, recombinant enzyme. 35% loss of activity after 2 h, 96% loss of activity after 27 h Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.1 4
-
 native enzyme Methanothermobacter thermautotrophicus
1.5.98.1 4.7
-
 recombinant enzyme Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 coenzyme F420 required Methanothermobacter thermautotrophicus