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Literature summary extracted from

  • Curien, G.; Job, D.; Douce, R.; Dumas, R.
    Allosteric activation of Arabidopsis threonine synthase by S-adenosylmethionine (1998), Biochemistry, 37, 13212-13221.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.1 S-adenosylmethionine affects both Km and Vmax by allosteric and cooperative transition of enzyme, two mol per mol enzyme, 25fold decrease of Km at 0.06 mM Arabidopsis thaliana

Application

EC Number Application Comment Organism
4.2.3.1 agriculture possible herbicide target Arabidopsis thaliana
4.2.3.1 nutrition interesting with respect to attempts to obtain transgenic plants with elevated levels of essential amino acids Met, Lys, Thr Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.1 expressed in Escherichia coli Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.1 110000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.1 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.1 recombinant enzyme Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.1 O-phospho-L-homoserine + H2O = L-threonine + phosphate mechanism Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.1 3.8
-
-
 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
4.2.3.1 -80°C, Na-HEPES buffer, pH 7.5, several months, no loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.1 O-phospho-L-homoserine + H2O
-
 Arabidopsis thaliana L-threonine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.1 dimer
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.1 30
-
 enzyme assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.1 0.4
-
 O-phosphohomoserine without S-adenosylmethionine Arabidopsis thaliana
4.2.3.1 3.5
-
 O-phosphohomoserine at 0.06 mM S-adenosylmethionine Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.1 8
-
 enzyme assay at Arabidopsis thaliana