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Literature summary extracted from

  • Laber, B.; Gerbling, K.P.; Harde, C.; Neff, K.H.; Nordhoff, E.; Pohlenz, H.D.
    Mechanisms of interaction of Escherichia coli threonine synthase with substrates and inhibitors (1994), Biochemistry, 33, 3413-3423.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.1 DL-2-amino-3[(phosphonomethyl)thio]propionic acid Ki: 0.057 mM, kinact: 1.44 min-1 Escherichia coli
4.2.3.1 DL-E-2-amino-5-phosphono-4-pentenoic acid Ki: 0.54 mM Escherichia coli
4.2.3.1 L-2,3-methanohomoserine phosphate Ki: 0.01 mM Escherichia coli
4.2.3.1 L-2-amino-3[(phosphonomethyl)thio]propionic acid Ki: 0.011 mM Escherichia coli
4.2.3.1 L-3-hydroxyhomoserine Ki: 0.05 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.1 0.5
-
 O-phosphohomoserine
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.1 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.1 O-phospho-L-homoserine + H2O = L-threonine + phosphate mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.1 DL-3-chloroalanine beta-elimination Escherichia coli pyruvate + NH3 + HCl
-
 ir
4.2.3.1 DL-vinylglycine + H2O
-
 Escherichia coli L-threonine
-
 ir
4.2.3.1 L-allo-threonine + H2O beta-elimination Escherichia coli 2-oxobutyrate + NH3
-
 ir
4.2.3.1 L-serine beta-elimination Escherichia coli pyruvate + NH3
-
 ir
4.2.3.1 additional information half-transamination reactions Escherichia coli ?
-
 ?
4.2.3.1 threonine beta-elimination Escherichia coli 2-oxobutyrate + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.1 7.33
-
 O-phosphohomoserine
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.3.1 0.01
-
 L-2,3-methanohomoserine phosphate
-
 Escherichia coli
4.2.3.1 0.011
-
 L-2-amino-3[(phosphonomethyl)thio]propionic acid
-
 Escherichia coli
4.2.3.1 0.05
-
 L-3-hydroxyhomoserine
-
 Escherichia coli
4.2.3.1 0.057
-
 DL-2-amino-3[(phosphonomethyl)thio]propionic acid kinact: 1.44 min-1 Escherichia coli
4.2.3.1 0.54
-
 DL-E-2-amino-5-phosphono-4-pentenoic acid
-
 Escherichia coli